ArticleAmerican journal of physiology. Cell physiology2009
Role of p47(phox) in regulating Cdc42GAP, vimentin, and contraction in smooth muscle cells.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
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- p21-activated kinase 1 (PAK1) as a therapeutic target for cardiotoxicity.Archives of toxicology · 2022Review
- Regulation of smooth muscle contraction by monomeric non-RhoA GTPases.British journal of pharmacology · 2020Review
- Rho and Reactive Oxygen Species at Crossroads of Endothelial Permeability and Inflammation.Antioxidants & redox signaling · 2019Article
- Reorganization of the Vimentin Network in Smooth Muscle.Journal of engineering and science in medical diagnostics and therapy · 2019Article
- The biological impact of blood pressure-associated genetic variants in the natriuretic peptide receptor C gene on human vascular smooth muscle.Human molecular genetics · 2018Article
- Polo-like Kinase 1 Regulates Vimentin Phosphorylation at Ser-56 and Contraction in Smooth Muscle.The Journal of biological chemistry · 2016Article
- Vimentin dephosphorylation at ser-56 is regulated by type 1 protein phosphatase in smooth muscle.Respiratory research · 2016Article
- Flavone inhibits migration through DLC1/RhoA pathway by decreasing ROS generation in breast cancer cells.In vitro cellular & developmental biology. Animal · 2016Article
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- Critical role of actin-associated proteins in smooth muscle contraction, cell proliferation, airway hyperresponsiveness and airway remodeling.Respiratory research · 2015Review
- The role of reactive oxygen species in microvascular remodeling.International journal of molecular sciences · 2014Review
- c-Abl tyrosine kinase regulates cytokinesis of human airway smooth muscle cells.American journal of respiratory cell and molecular biology · 2014Article
- The association of cortactin with profilin-1 is critical for smooth muscle contraction.The Journal of biological chemistry · 2014Article
- Role of the adapter protein Abi1 in actin-associated signaling and smooth muscle contraction.The Journal of biological chemistry · 2013Article
- Raf-1, actin dynamics, and abelson tyrosine kinase in human airway smooth muscle cells.American journal of respiratory cell and molecular biology · 2013Article
- Abl regulates smooth muscle cell proliferation by modulating actin dynamics and ERK1/2 activation.American journal of physiology. Cell physiology · 2012Article
- Cigarette Smoke Extract-induced Reduction in Migration and Contraction in Normal Human Bronchial Smooth Muscle Cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2011Article
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2 authors at 1 institution in 1 country.
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Abstract
Cdc42GAP (GTPase activating protein) has been shown to regulate smooth muscle contraction as well as cell motility, adhesion, proliferation, and apoptosis. We have recently shown that Cdc42GAP activity is suppressed in smooth muscle cells during contractile activation, which is reversed by inhibitors of reactive oxygen species (ROS). Because p47(phox), a regulatory subunit of NAD(P)H oxidase, has been implicated in smooth muscle signaling, we determined whether this subunit modulates Cdc42GAP activity in response to contractile stimulation. Transfection of smooth muscle cells with plasmids encoding short hairpin RNA (shRNA) against p47(phox), but not plasmids for luciferase shRNA, inhibited the expression of p47(phox). ROS production and the suppression of Cdc42GAP activity in response to stimulation with 5-hydroxytryptamine (5-HT) were attenuated in cells producing p47(phox) shRNA compared with cells producing luciferase shRNA. In contrast, the addition of hydrogen peroxide to p47(phox)-deficient cells suppressed the activity of Cdc42GAP. Furthermore, exposure to hydrogen peroxide led to a decrease in Cdc42GAP activity in an in vitro assay. Cdc42 activation, p21-activated kinase 1 (PAK1) phosphorylation at Thr-423 (an indication of PAK activation), and vimentin phosphorylation at Ser-56 in response to 5-HT activation were also attenuated in smooth muscle cells producing shRNA against p47(phox). The knockdown of p47(phox) inhibited smooth muscle contraction during stimulation with 5-HT but not hydrogen peroxide. These results suggest that the p47(phox) subunit of NAD(P)H oxidase may mediate the agonist-induced GAP suppression by controlling ROS generation in smooth muscle cells during agonist stimulation. p47(phox)-regulated GAP affects smooth muscle contraction likely through the Cdc42/PAK1/vimentin pathway.
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