ArticleMolecular biology of the cell2009
Utp9p facilitates Msn5p-mediated nuclear reexport of retrograded tRNAs in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Gle1 is required for tRNA to stimulate Dbp5 ATPase activitybioRxiv : the preprint server for biology · 2023Article
- Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing.RNA biology · 2023Article
- Prediction of Protein Subcellular Localization Based on Fusion of Multi-view Features.Molecules (Basel, Switzerland) · 2019Article
- Retrograde nuclear transport from the cytoplasm is required for tRNARNA biology · 2018Article
- Nup100 regulatesRNA (New York, N.Y.) · 2017Article
- Cytosolic Hsp70 and co-chaperones constitute a novel system for tRNA import into the nucleus.eLife · 2015Article
- RNA Export through the NPC in Eukaryotes.Genes · 2015Review
- Protein kinase A is part of a mechanism that regulates nuclear reimport of the nuclear tRNA export receptors Los1p and Msn5p.Eukaryotic cell · 2014Article
- In vitro reconstitution of yeast tUTP/UTP A and UTP B subcomplexes provides new insights into their modular architecture.PloS one · 2014Article
- Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- Nuclear-cytoplasmic trafficking of NTF2, the nuclear import receptor for the RanGTPase, is subjected to regulation.PloS one · 2012Article
- Transportin 3 promotes a nuclear maturation step required for efficient HIV-1 integration.PLoS pathogens · 2011Article
- Plants, like mammals, but unlike Saccharomyces, do not regulate nuclear-cytoplasmic tRNA trafficking in response to nutrient stress.Plant signaling & behavior · 2011Article
- P-body components, Dhh1 and Pat1, are involved in tRNA nuclear-cytoplasmic dynamics.RNA (New York, N.Y.) · 2011Article
- Nutrient stress does not cause retrograde transport of cytoplasmic tRNA to the nucleus in evolutionarily diverse organisms.Molecular biology of the cell · 2011Article
- tRNA biology charges to the front.Genes & development · 2010Review
- Scyl1 facilitates nuclear tRNA export in mammalian cells by acting at the nuclear pore complex.Molecular biology of the cell · 2010Article
- The ins and outs of nuclear re-export of retrogradely transported tRNAs in Saccharomyces cerevisiae.Nucleus (Austin, Tex.)Review
- Transfer RNA travels from the cytoplasm to organelles.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Utp9p is a nucleolar protein that is part of a subcomplex containing several U3 snoRNA-associated proteins including Utp8p, which is a protein that shuttles aminoacyl-tRNAs from the nucleolus to the nuclear tRNA export receptors Los1p and Msn5p in Saccharomyces cerevisiae. Here we show that Utp9p is also an intranuclear component of the Msn5p-mediated nuclear tRNA export pathway. Depletion of Utp9p caused nuclear accumulation of mature tRNAs derived from intron-containing precursors, but not tRNAs made from intronless pre-tRNAs. Utp9p binds tRNA directly and saturably, and copurifies with Utp8p, Gsp1p, and Msn5p, but not with Los1p or aminoacyl-tRNA synthetases. Utp9p interacts directly with Utp8p, Gsp1p, and Msn5p in vitro. Furthermore, Gsp1p forms a complex with Msn5p and Utp9p in a tRNA-dependent manner. However, Utp9p does not shuttle between the nucleus and the cytoplasm. Because tRNA splicing occurs in the cytoplasm and the spliced tRNAs are retrograded back to the nucleus, we propose that Utp9p facilitates nuclear reexport of retrograded tRNAs. Moreover, the data suggest that Utp9p together with Utp8p translocate aminoacyl-tRNAs from the nucleolus to Msn5p and assist with formation of the Msn5p-tRNA-Gsp1p-GTP export complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.