ArticlePlant physiology2009
In situ mapping of nutrient uptake in the rhizosphere using nanoscale secondary ion mass spectrometry.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Integrating soil imaging with spatial omics to uncover root-soil interactions.Plant communications · 2026Review
- Engineered Nanomaterials, Microbial Community Responses, and Fe-Mediated Regulation of As and Cd Fate in the Flooded Rice Rhizosphere: A Mechanistic Synthesis.Microorganisms · 2026Review
- Resolving subcellular sucrose concentrations in plant tissues.Journal of experimental botany · 2026Review
- Linking micro-X-ray fluorescence spectroscopy and X-ray computed tomography with model simulation explains differences in nutrient gradients around roots of different types and ages.The New phytologist · 2025Article
- Gold-FISH enables targeted NanoSIMS analysis of plant-associated bacteria.The New phytologist · 2023Article
- Mass Spectral Imaging to Map Plant-Microbe Interactions.Microorganisms · 2023Review
- In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor.Science advances · 2022Article
- Review
- Zinc and Cadmium Mapping in the Apical Shoot and Hypocotyl Tissues of Radish by High-Resolution Secondary Ion Mass Spectrometry (NanoSIMS) after Short-Term Exposure to Metal Contamination.International journal of environmental research and public health · 2019Article
- Engineering abiotic stress response in plants for biomass production.The Journal of biological chemistry · 2018Review
- The emergent rhizosphere: imaging the development of the porous architecture at the root-soil interface.Scientific reports · 2017Article
- Quantitative imaging of deuterated metabolic tracers in biological tissues with nanoscale secondary ion mass spectrometry.International journal of mass spectrometry · 2017Article
- Article
- Multi-scale imaging of anticancer platinum(iv) compounds in murine tumor and kidney.Chemical science · 2016Article
- Advancements in the application of NanoSIMS and Raman microspectroscopy to investigate the activity of microbial cells in soils.FEMS microbiology ecology · 2015Article
- Exploring the transfer of recent plant photosynthates to soil microbes: mycorrhizal pathway vs direct root exudation.The New phytologist · 2015Article
- Three-Dimensional Mapping of Soil Chemical Characteristics at Micrometric Scale by Combining 2D SEM-EDX Data and 3D X-Ray CT Images.PloS one · 2015Article
- Microbial community dynamics alleviate stoichiometric constraints during litter decay.Ecology letters · 2014Article
- Emerging mass spectrometry techniques for the direct analysis of microbial colonies.Current opinion in microbiology · 2014Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant roots and microorganisms interact and compete for nutrients within the rhizosphere, which is considered one of the most biologically complex systems on Earth. Unraveling the nitrogen (N) cycle is key to understanding and managing nutrient flows in terrestrial ecosystems, yet to date it has proved impossible to analyze and image N transfer in situ within such a complex system at a scale relevant to soil-microbe-plant interactions. Linking the physical heterogeneity of soil to biological processes marks a current frontier in plant and soil sciences. Here we present a new and widely applicable approach that allows imaging of the spatial and temporal dynamics of the stable isotope (15)N assimilated within the rhizosphere. This approach allows visualization and measurement of nutrient resource capture between competing plant cells and microorganisms. For confirmation we show the correlative use of nanoscale secondary ion mass spectrometry, and transmission electron microscopy, to image differential partitioning of (15)NH(4)(+) between plant roots and native soil microbial communities at the submicron scale. It is shown that (15)N compounds can be detected and imaged in situ in individual microorganisms in the soil matrix and intracellularly within the root. Nanoscale secondary ion mass spectrometry has potential to allow the study of assimilatory processes at the submicron level in a wide range of applications involving plants, microorganisms, and animals.
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