Evidence map›Paper›PMID 19809889›Full record

ArticleMolecular biology reports2010

Characteristics of X- and Y-chromosome specific regions of the amelogenin gene and a PCR-based method for sex identification in red deer (Cervus elaphus).

Artur Gurgul, Anna Radko, Ewa Słota

Abstract read
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In one paragraph

Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 23 citations in OpenAlex.

  1. Development of a tetra-primer ARMS-PCR for identification of sika and red deer and their hybrids.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Artur GurgulDepartment of Animal Immuno- and Cytogenetics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice n., Krakow, Poland. arturgurgul@wp.pl
Anna Radko
Ewa Słota
National Research Institute of Animal Production · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study attempts to analyse sequences of the X- and Y-chromosome specific regions of the amelogenin (AMEL) gene in red deer. To this end, primers specific for each form of the gene (AMELX and AMELY) were designed based on bovine genomic sequences and the homologous regions of the genes were sequenced. The obtained sequence of AMELX gene showed high similarity with the corresponding region in cattle (91%) and humans (77%), but this similarity was slightly lower among AMELY genes and showed 87 and 73% of identical nucleotides, respectively. In addition, three single nucleotide polymorphisms (SNPs) were found in the AMELX gene of the female red deer investigated. Comparative analysis of the homologous fragments of the red deer AMELX and AMELY genes confirmed the deletion of an AMELY gene fragment in relation to AMELX. Homology of both sequences was 82% of identical nucleotides in the coding region and 74% in 3' non-coding sequence. The sequences studied showed considerable similarity to homologous fragments of the human and bovine gene, but the structural differences observed lead us to design PCR-based method for sex identification in red deer, based on the presented sequences.

Indexed as

AllelesAmelogeninAnimalsCattleDeerElectrophoresis, Agar GelFemaleGenotypeHumansMalePolymerase Chain ReactionPolymorphism, Single NucleotideSex Determination AnalysisX ChromosomeY ChromosomeAmelogenin

Identifiers

PMID19809889
OpenAlexW1980086475

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.