ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- High-throughput functional screen identifies YWHAZ as a key regulator of pancreatic cancer metastasis.Cell death & disease · 2023Article
- Genome Engineering of Human Urine-Derived Stem Cells to Express Lactoferrin and Deoxyribonuclease.Tissue engineering. Part A · 2023Article
- Applications ofStem cells international · 2021Review
- Adeno-Associated Virus-Based Gene Therapy for Lifelong Correction of Genetic Disease.Human gene therapy · 2020Review
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- Genome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function.Cellular and molecular bioengineering · 2020Article
- In VivoInternational journal of molecular sciences · 2019Article
- A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells.Cell communication and signaling : CCS · 2019Article
- Liver Cancer Gene Discovery Using Gene Targeting, Sleeping Beauty, and CRISPR/Cas9.Seminars in liver disease · 2019Review
- Direct reprogramming to human nephron progenitor-like cells using inducible piggyBac transposon expression of SNAI2-EYA1-SIX1.Kidney international · 2019Article
- Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-DirectedStem cells international · 2019Review
- Intravenous Delivery ofInternational journal of molecular sciences · 2018Article
- Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo.Nature communications · 2018Article
- Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.Nucleic acids research · 2018Article
- B cell-derived IL-4 acts on podocytes to induce proteinuria and foot process effacement.JCI insight · 2017Article
- Transposase-mediated gene modulation in the placenta.Placenta · 2017Review
- One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes.Molecular therapy. Nucleic acids · 2017Article
- Kidney-specific transposon-mediated gene transfer in vivo.Scientific reports · 2017Article
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Authors and funding
7 authors.
Funding
Abstract
Somatic cell gene transfer has permitted inducible gene expression in vivo through coinfection of multiple viruses. We hypothesized that the highly efficient plasmid-based piggyBac transposon system would enable long-term inducible gene expression in mice in vivo. We used a multiple-transposon delivery strategy to create a tetracycline-inducible expression system in vitro in human cells by delivering the two genes on separate transposons for inducible reporter gene expression along with a separate selectable transposon marker. Evaluation of stable cell lines revealed 100% of selected clones exhibited inducible expression via stable expression from three separate transposons simultaneously. We next tested and found that piggyBac-mediated gene transfer to liver or lung could achieve stable reporter gene expression in mice in vivo in either immunocompetent or immune deficient animals. A single injection of piggyBac transposons could achieve long-term inducible gene expression in the livers of mice in vivo, confirming our multiple-transposon strategy used in cultured cells. The plasmid-based piggyBac transposon system enables constitutive or inducible gene expression in vivo for potential therapeutic and biological applications without using viral vectors.
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