ArticleJournal of cell science2009
Real-time measurements of cAMP production in live Dictyostelium cells.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- From microscope to model: rotating signaling dynamics and cluster size in Dictyostelium discoideum aggregates.BMC molecular and cell biology · 2026Article
- Establishing functional giant Dictyostelium cells reveals front-rear polarity in intracellular signaling.Communications biology · 2026Article
- Article
- Visualization of c-di-GMP in multicellularFrontiers in cell and developmental biology · 2023Article
- Review
- Analysis of barotactic and chemotactic guidance cues on directional decision-making ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Oscillatory cAMP cell-cell signalling persists during multicellularCommunications biology · 2019Article
- Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals.The Journal of cell biology · 2018Article
- Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP inJournal of cell science · 2017Article
- From intracellular signaling to population oscillations: bridging size- and time-scales in collective behavior.Molecular systems biology · 2015Article
- Distinct cell shapes determine accurate chemotaxis.Scientific reports · 2013Article
- Modeling and measuring signal relay in noisy directed migration of cell groups.PLoS computational biology · 2013Article
- Luminescent proteins for high-speed single-cell and whole-body imaging.Nature communications · 2012Article
- Direct biochemical measurements of signal relay during Dictyostelium development.The Journal of biological chemistry · 2011Article
- mTORC2 regulates neutrophil chemotaxis in a cAMP- and RhoA-dependent fashion.Developmental cell · 2010Article
- Approaching the molecular origins of collective dynamics in oscillating cell populations.Current opinion in genetics & development · 2010Review
- The exocytic gene secA is required for Dictyostelium cell motility and osmoregulation.Journal of cell science · 2010Article
- Live cell imaging of mechanotransduction.Journal of the Royal Society, Interface · 2010Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Cyclic AMP has a crucial role during the entire developmental program of the social amoebae Dictyostelium, acting both as an intracellular second messenger and, when secreted, as a directional cue that is relayed to neighboring cells during chemotaxis. Although significant knowledge about cAMP production in chemotaxing cells has been derived from studies performed on cell populations, cAMP dynamics at the single cell level have not been investigated. To examine this, we used a FRET-based cAMP sensor that possesses high cAMP sensitivity and great temporal resolution. We show the transient profile of cAMP accumulation in live Dictyostelium cells and establish that chemoattractants control intracellular cAMP dynamics by regulating synthesis via the adenylyl cyclase ACA. aca(-) cells show no significant change in FRET response following chemoattractant addition. Furthermore, cells lacking ACB, the other adenylyl cyclase expressed in chemotaxing cells, behave similarly to wild-type cells. We also establish that the RegA is the major phosphodiesterase that degrades intracellular cAMP in chemotaxis-competent cells. Interestingly, we failed to measure intracellular cAMP compartmentalization in actively chemotaxing cells. We conclude that cytosolic cAMP, which is destined to activate PKA, is regulated by ACA and RegA and does not compartmentalize during chemotaxis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.