ArticleJournal of the American Chemical Society2009
Crystal structures of penicillin-binding protein 6 from Escherichia coli.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Molecular Insights into the β‑Lactam α‑Methyl Modulation ofACS omega · 2026Article
- Natural Product-Derived Drugs: Structural Insights into Their Biological Mechanisms.Biomolecules · 2025Review
- Structural Insights for β-Lactam Antibiotics.Biomolecules & therapeutics · 2023Review
- Structural Insights into Substrate Binding and Antibiotic Inhibition of Enterobacterial Penicillin-Binding Protein 6.Life (Basel, Switzerland) · 2022Article
- A strategy for mitigating avian colibacillosis disease using plant growth promoting rhizobacteria and green synthesized zinc oxide nanoparticles.Saudi journal of biological sciences · 2021Article
- Influence of the α-Methoxy Group on the Reaction of Temocillin with Pseudomonas aeruginosa PBP3 and CTX-M-14 β-Lactamase.Antimicrobial agents and chemotherapy · 2019Article
- Synthesis and biological evaluation of zinc chelating compounds as metallo-β-lactamase inhibitors.MedChemComm · 2019Article
- The putative polysaccharide deacetylase Ba0331: cloning, expression, crystallization and structure determination.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Structural and kinetic analyses of penicillin-binding protein 4 (PBP4)-mediated antibiotic resistance inThe Journal of biological chemistry · 2018Article
- Crystal Structures of Penicillin-Binding Protein D2 from Listeria monocytogenes and Structural Basis for Antibiotic Specificity.Antimicrobial agents and chemotherapy · 2018Article
- The low-molecular-mass, penicillin-binding proteins DacB and DacC combine to modify peptidoglycan cross-linking and allow stable Type IV pilus expression in Neisseria gonorrhoeae.Molecular microbiology · 2018Article
- Article
- Biochemical and Structural Analysis of a Novel Esterase from Caulobacter crescentus related to Penicillin-Binding Protein (PBP).Scientific reports · 2016Article
- In vivo functional and molecular characterization of the Penicillin-Binding Protein 4 (DacB) of Pseudomonas aeruginosa.BMC microbiology · 2016Article
- Article
- Two Putative Polysaccharide Deacetylases Are Required for Osmotic Stability and Cell Shape Maintenance in Bacillus anthracis.The Journal of biological chemistry · 2015Article
- Protonation states of active-site lysines of penicillin-binding protein 6 from Escherichia coli and the mechanistic implications.Proteins · 2014Article
- Crystallization and preliminary X-ray crystallographic analysis of PBPD2 from Listeria monocytogenes.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Subfamily-specific adaptations in the structures of two penicillin-binding proteins from Mycobacterium tuberculosis.PloS one · 2014Article
- Structural basis for the broad specificity of a new family of amino-acid racemases.Acta crystallographica. Section D, Biological crystallography · 2014Article
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7 authors.
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Abstract
Penicillin-binding protein 6 (PBP6) is one of the two main DD-carboxypeptidases in Escherichia coli, which are implicated in maturation of bacterial cell wall and formation of cell shape. Here, we report the first X-ray crystal structures of PBP6, capturing its apo state (2.1 A), an acyl-enzyme intermediate with the antibiotic ampicillin (1.8 A), and for the first time for a PBP, a preacylation complex (a "Michaelis complex", determined at 1.8 A) with a peptidoglycan substrate fragment containing the full pentapeptide, NAM-(L-Ala-D-isoGlu-L-Lys-D-Ala-D-Ala). These structures illuminate the molecular interactions essential for ligand recognition and catalysis by DD-carboxypeptidases, and suggest a coupling of conformational flexibility of active site loops to the reaction coordinate. The substrate fragment complex structure, in particular, provides templates for models of cell wall recognition by PBPs, as well as substantiating evidence for the molecular mimicry by beta-lactam antibiotics of the peptidoglycan acyl-D-Ala-D-Ala moiety.
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