ArticleJournal of the American Chemical Society2009
An electrochemical sensor for single nucleotide polymorphism detection in serum based on a triple-stem DNA probe.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Constant Temperature Electrochemical Biosensor for SNP Detection in Human Genomic DNA Based on DNA Melting Analysis.ACS sensors · 2025Article
- Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- Electrochemical Impedimetric Study of Non-Watson-Crick Base Pairs of DNA.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Utilization of chromogenic enzyme substrates for signal amplification in multiplexed detection of biomolecules using surface mass spectrometry.Sensors and actuators. B, Chemical · 2021Article
- Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors.Nano letters · 2020Article
- Microfluidic Technologies for cfDNA Isolation and Analysis.Micromachines · 2019Review
- Design of DNA nanostructure-based interfacial probes for the electrochemical detection of nucleic acids directly in whole blood.Chemical science · 2018Article
- A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS).The Analyst · 2016Article
- Highly specific SNP detection using 2D graphene electronics and DNA strand displacement.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Accurate zygote-specific discrimination of single-nucleotide polymorphisms using microfluidic electrochemical DNA melting curves.Angewandte Chemie (International ed. in English) · 2014Article
- DNA electrochemistry with tethered methylene blue.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method.International journal of nanomedicine · 2012Article
- Fabrication of electrochemical-DNA biosensors for the reagentless detection of nucleic acids, proteins and small molecules.Journal of visualized experiments : JoVE · 2011Article
- Selective fluorescence sensing of deoxycytidine 5'-monophosphate (dCMP) employing a bis(diphenylphosphate)diimine ligand.Journal of fluorescence · 2011Article
- Toward a general approach for RNA-templated hierarchical assembly of split-proteins.Journal of the American Chemical Society · 2010Article
- Electrochemical sensors.Analytical chemistry · 2010Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We report here an electrochemical approach that offers, for the first time, single-step, room-temperature single nucleotide polymorphism (SNP) detection directly in complex samples (such as blood serum) without the need for target modification, postwashing, or the addition of exogenous reagents. This sensor, which is sensitive, stable, and reusable, is comprised of a single, self-complementary, methylene blue-labeled DNA probe possessing a triple-stem structure. This probe takes advantage of the large thermodynamic changes in enthalpy and entropy that result from major conformational rearrangements that occur upon binding a perfectly matched target, resulting in a large-scale change in the faradaic current. As a result, the discrimination capabilities of this sensor greatly exceed those of earlier single- and double-stem electrochemical sensors and support rapid (minutes), single-step, reagentless, room-temperature detection of single nucleotide substitutions. To elucidate the theoretical basis of the sensor's selectivity, we present a comparative thermodynamic analysis among single-, double-, and triple-stem probes.
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Registered trials
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