ArticleGenetics2009
DLA-based strategies for cloning insertion mutants: cloning the gl4 locus of maize using Mu transposon tagged alleles.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Transposon-based genome editing of industrial microorganisms: advances, challenges, and prospects.Synthetic and systems biotechnology · 2026Review
- Large DNA and protein language models enhance discovery of deleterious mutations in maize.Genome biology · 2025Article
- Genetic interaction between GL15 and FDL1 modulates juvenile cuticle deposition and leaf permeability in maize.Journal of experimental botany · 2025Article
- Understanding Callus Types in Maize by Genetic Mapping and Transcriptional Profiling.Plants (Basel, Switzerland) · 2025Article
- Transcriptome profiling of maize transcription factor mutants to probe gene regulatory network predictions.G3 (Bethesda, Md.) · 2025Article
- Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.Journal of experimental botany · 2024Article
- Integrative multi-omic analysis identifies genes associated with cuticular wax biogenesis in adult maize leaves.G3 (Bethesda, Md.) · 2024Article
- Bacterium-enabled transient gene activation by artificial transcription factors for resolving gene regulation in maize.The Plant cell · 2023Article
- Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii.Nature communications · 2022Article
- Drought-ResponsivePlant physiology · 2020Article
- Maize glossy6 is involved in cuticular wax deposition and drought tolerance.Journal of experimental botany · 2019Article
- Article
- Advances in the understanding of cuticular waxes in Arabidopsis thaliana and crop species.Plant cell reports · 2015Review
- Article
- Identification of the maize gravitropism gene lazy plant1 by a transposon-tagging genome resequencing strategy.PloS one · 2014Article
- Article
- Article
- Genome walking by next generation sequencing approaches.Biology · 2012Article
- PPR2263, a DYW-Subgroup Pentatricopeptide repeat protein, is required for mitochondrial nad5 and cob transcript editing, mitochondrion biogenesis, and maize growth.The Plant cell · 2012Article
- Gene mapping via bulked segregant RNA-Seq (BSR-Seq).PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digestion-ligation-amplification (DLA), a novel adaptor-mediated PCR-based method that uses a single-stranded oligo as the adaptor, was developed to overcome difficulties of amplifying unknown sequences flanking known DNA sequences in large genomes. DLA specifically overcomes the problems associated with existing methods for amplifying genomic sequences flanking Mu transposons, including high levels of nonspecific amplification. Two DLA-based strategies, MuClone and DLA-454, were developed to isolate Mu-tagged alleles. MuClone allows for the amplification of subsets of the numerous Mu transposons in the genome, using unique three-nucleotide tags at the 3' ends of primers, simplifying the identification of flanking sequences that cosegregate with mutant phenotypes caused by Mu insertions. DLA-454, which combines DLA with 454 pyrosequencing, permits the efficient cloning of genes for which multiple independent insertion alleles are available without the need to develop segregating populations. The utility of each approach was validated by independently cloning the gl4 (glossy4) gene. Mutants of gl4 lack the normal accumulation of epicuticular waxes. The gl4 gene is a homolog of the Arabidopsis CUT1 gene, which encodes a condensing enzyme involved in the synthesis of very-long-chain fatty acids, which are precursors of epicuticular waxes.
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