ArticleThe Journal of cell biology2009
Relative contributions of chromatin and kinetochores to mitotic spindle assembly.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 86 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation.PLoS biology · 2024Article
- Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity.Nature communications · 2023Article
- Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression.Current biology : CB · 2023Article
- Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly.Nature communications · 2022Article
- Article
- Polar Chromosomes-Challenges of a Risky Path.Cells · 2022Review
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- Role of spatial patterns and kinetochore architecture in spindle morphogenesis.Seminars in cell & developmental biology · 2021Review
- Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in oocytes.EMBO reports · 2021Article
- Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.Nature communications · 2020Article
- RanGTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes.The EMBO journal · 2020Article
- Review
- BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules.Cell research · 2019Article
- Microtubules assemble near most kinetochores during early prometaphase in human cells.The Journal of cell biology · 2018Article
- Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments.Nature communications · 2018Article
- Kinesin-5 mediated chromosome congression in insect spindles.Cellular and molecular bioengineering · 2018Article
- Delivery of Native Proteins into C. elegans Using a Transduction Protocol Based on Lipid Vesicles.Scientific reports · 2017Article
- The chromokinesin Klp3a and microtubules facilitate acentric chromosome segregation.The Journal of cell biology · 2017Article
- C. elegans chromosomes connect to centrosomes by anchoring into the spindle network.Nature communications · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
During mitosis and meiosis in animal cells, chromosomes actively participate in spindle assembly by generating a gradient of Ran guanosine triphosphate (RanGTP). A high concentration of RanGTP promotes microtubule nucleation and stabilization in the vicinity of chromatin. However, the relative contributions of chromosome arms and centromeres/kinetochores in this process are not known. In this study, we address this issue using cells undergoing mitosis with unreplicated genomes (MUG). During MUG, chromatin is rapidly separated from the forming spindle, and both centrosomal and noncentrosomal spindle assembly pathways are active. MUG chromatin is coated with RCC1 and establishes a RanGTP gradient. However, a robust spindle forms around kinetochores/centromeres outside of the gradient peak. When stable kinetochore microtubule attachment is prevented by Nuf2 depletion in both MUG and normal mitosis, chromatin attracts astral microtubules but cannot induce spindle assembly. These results support a model in which kinetochores play the dominant role in the chromosome-mediated pathway of mitotic spindle assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.