ArticleAntimicrobial agents and chemotherapy2010
Clindamycin-induced CovS-mediated regulation of the production of virulent exoproteins streptolysin O, NAD glycohydrolase, and streptokinase in Streptococcus pyogenes.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- A comprehensive analysis of antimicrobial resistance of clinicalJAC-antimicrobial resistance · 2025Article
- Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treatScience advances · 2024Article
- Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treatbioRxiv : the preprint server for biology · 2024Article
- Association between adjunct clindamycin and in-hospital mortality in patients with necrotizing soft tissue infection due to group A Streptococcus: a nationwide cohort study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022Article
- General Phenotype of NADase Induction by CLI Treatment inInternational journal of microbiology · 2022Article
- Effect of Phosphatase Activity of the Control of Virulence Sensor (CovS) on Clindamycin-Mediated Streptolysin O Production in Group AInfection and immunity · 2019Article
- Molecular characterization of the 2011 Hong Kong scarlet fever outbreak.The Journal of infectious diseases · 2012Article
- Streptococcus pyogenes and re-emergence of scarlet fever as a public health problem.Emerging microbes & infections · 2012Review
- Counteractive balancing of transcriptome expression involving CodY and CovRS in Streptococcus pyogenes.Journal of bacteriology · 2011Article
- Protective effect of hainosankyuto, a traditional Japanese medicine, on Streptococcus pyogenes infection in murine model.PloS one · 2011Article
- The Streptococcus pyogenes proteome: maps, virulence factors and vaccine candidates.Future microbiology · 2010Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The administration of high-dose clindamycin (CLI) along with penicillin is recommended for the treatment of streptococcal toxic shock syndrome. However, the prevalence of CLI-resistant Streptococcus pyogenes strains is increasing worldwide, and the effect of CLI on CLI-resistant S. pyogenes strains remains unknown. We aimed to evaluate the effect of CLI on the in vitro production of three major virulent exoproteins, namely, streptolysin O (Slo), NAD glycohydrolase (Nga), and streptokinase (Ska), by CLI-resistant S. pyogenes strains. After the incubation of M1 serotype CLI-resistant S. pyogenes D2TY in the presence of 1 microg/ml CLI, the amounts of Slo, Nga, and Ska and the levels of slo, nga, and ska mRNA in the supernatant were analyzed by Northern blotting and Western blotting, respectively. The results of both assays showed that the production of Slo, Nga, and Ska was higher with CLI treatment than without CLI treatment. We evaluated the role of the sensor kinase CovS, which is involved in the two-component system of S. pyogenes, in the CLI-induced production of these three exoproteins. Northern blotting analysis revealed that CLI induced the expression of covS mRNA in wild-type strain D2TY. Furthermore, both Northern blotting and Western blotting analyses showed that CLI decreased the levels of expression of Slo, Nga, and Ska in isogenic covS mutant D2TYcovS. These results suggest that CLI increases the production of three virulent exoproteins in CLI-resistant S. pyogenes strains via the action of CovS.
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