ArticleProceedings of the National Academy of Sciences of the United States of America2009
Mediator complex association with constitutively transcribed genes in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 74 citations in OpenAlex.
- Intergenic accumulation of RNA polymerase II maintains the potential for swift transcriptional restart upon release from quiescence.Genome research · 2025Article
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- The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes.Molecular biology of the cell · 2025Article
- Growth-regulated co-occupancy of Mediator and Lsm3 at intronic ribosomal protein genes.Nucleic acids research · 2024Article
- Mediator dynamics during heat shock in budding yeast.Genome research · 2022Article
- Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.Molecular cell · 2021Article
- Mediator Subunits MED16, MED14, and MED2 Are Required for Activation of ABRE-Dependent Transcription in Arabidopsis.Frontiers in plant science · 2021Article
- Article
- Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes.Genes & development · 2018Article
- Candida glabrata Med3 Plays a Role in Altering Cell Size and Budding Index To Coordinate Cell Growth.Applied and environmental microbiology · 2018Article
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- The Mediator co-activator complex regulates Ty1 retromobility by controlling the balance between Ty1i and Ty1 promoters.PLoS genetics · 2018Article
- SAGA Is a General Cofactor for RNA Polymerase II Transcription.Molecular cell · 2017Article
- Genome-wide characterization of Mediator recruitment, function, and regulation.Transcription · 2017Review
- Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.Transcription · 2017Review
- Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters.The EMBO journal · 2016Article
- Dual role of Med12 in PRC1-dependent gene repression and ncRNA-mediated transcriptional activation.Cell cycle (Georgetown, Tex.) · 2016Article
- On the way of revealing coactivator complexes cross-talk during transcriptional activation.Cell & bioscience · 2016Review
- The Mediator Complex Subunits MED14, MED15, and MED16 Are Involved in Defense Signaling Crosstalk inFrontiers in plant science · 2016Article
- Identification of MEDIATOR16 as the Arabidopsis COBRA suppressor MONGOOSE1.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mediator is a large, multisubunit complex that is essential for transcription of mRNA by RNA Pol II in eukaryotes and is believed to bridge transcriptional activators and the general transcription machinery. However, several recent studies suggest that the requirement for Mediator during transcriptional activation is not universal, but rather activator dependent, and may be indirect for some genes. Here we have investigated Mediator association with several constitutively transcribed genes in yeast by comparing a yeast strain that harbors a temperature-sensitive mutation in an essential Mediator subunit, Srb4, with its wild-type (WT) counterpart. We find modest association of Mediator with constitutively active genes and show that this association is strongly decreased in srb4 ts yeast, whereas association with a nontranscribed region or repressed gene promoters is lower and unaffected in the mutant yeast. The tail module of Mediator remains associated with ribosomal protein (RP) gene promoters in srb4 ts yeast, while subunits from the head and middle modules are lost. Tail module association at Rap1-dependent gene promoters is lost in rap1 ts yeast, indicating that Rap1 is required for Mediator recruitment at these gene promoters and that its recruitment occurs via the tail module. Pol II association is also rapidly and severely affected in srb4 ts yeast, indicating that Mediator is directly required for pol II association at constitutively transcribed genes. Our results are consistent with Mediator functioning as a general transcription factor in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.