ArticleProceedings of the National Academy of Sciences of the United States of America2009
Subcellular localization of a bacterial regulatory RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
- The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions.Cell reports · 2023Article
- The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions.bioRxiv : the preprint server for biology · 2023Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Mechanisms and consequences of subcellular RNA localization across diverse cell types.Traffic (Copenhagen, Denmark) · 2020Review
- BR-Bodies Provide Selectively Permeable Condensates that Stimulate mRNA Decay and Prevent Release of Decay Intermediates.Molecular cell · 2020Article
- More than just a phase: the search for membraneless organelles in the bacterial cytoplasm.Current genetics · 2019Review
- Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities.Frontiers in microbiology · 2019Review
- α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies.Molecular cell · 2018Article
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
- Non-coding RNAs Potentially Controlling Cell Cycle in the ModelFrontiers in genetics · 2018Article
- Nucleoid and cytoplasmic localization of small RNAs in Escherichia coli.Nucleic acids research · 2017Article
- The CsrA-FliW network controls polar localization of the dual-function flagellin mRNA in Campylobacter jejuni.Nature communications · 2016Article
- Article
- Genetic Selection of Peptide Aptamers That Interact and Inhibit Both Small Protein B and Alternative Ribosome-Rescue Factor A of Aeromonas veronii C4.Frontiers in microbiology · 2016Article
- Mechanisms of ribosome rescue in bacteria.Nature reviews. Microbiology · 2015Review
- Synthetic Biology: A Bridge between Artificial and Natural Cells.Life (Basel, Switzerland) · 2014Review
- Initiation of mRNA decay in bacteria.Cellular and molecular life sciences : CMLS · 2014Review
- RNA localization in bacteria.RNA biology · 2014Review
- Synergies between RNA degradation and trans-translation in Streptococcus pneumoniae: cross regulation and co-transcription of RNase R and SmpB.BMC microbiology · 2012Article
- Image analysis in fluorescence microscopy: bacterial dynamics as a case study.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotes and bacteria regulate the activity of some proteins by localizing them to discrete subcellular structures, and eukaryotes localize some RNAs for the same purpose. To explore whether bacteria also spatially regulate RNAs, the localization of tmRNA was determined using fluorescence in situ hybridization. tmRNA is a small regulatory RNA that is ubiquitous in bacteria and that interacts with translating ribosomes in a reaction known as trans-translation. In Caulobacter crescentus, tmRNA was localized in a cell-cycle-dependent manner. In G(1)-phase cells, tmRNA was found in regularly spaced foci indicative of a helix-like structure. After initiation of DNA replication, most of the tmRNA was degraded, and the remaining molecules were spread throughout the cytoplasm. Immunofluorescence assays showed that SmpB, a protein that binds tightly to tmRNA, was colocalized with tmRNA in the helix-like pattern. RNase R, the nuclease that degrades tmRNA, was localized in a helix-like pattern that was separate from the SmpB-tmRNA complex. These results suggest a model in which tmRNA-SmpB is localized to sequester tmRNA from RNase R, and localization might also regulate tmRNA-SmpB interactions with ribosomes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.