ArticleProceedings of the National Academy of Sciences of the United States of America2009
Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 216 citations in OpenAlex.
- Characterization of strain degeneration in the RL-P37 strain lineage of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2026Article
- Novel transcription factor regulating cellulase expression and growth improvement in cellulolytic fungi.Microbial cell factories · 2025Article
- Metagenomics-based tracing of genetically modified microorganism contaminations in commercial fermentation products.Food chemistry. Molecular sciences · 2025Article
- Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw.Bioprocess and biosystems engineering · 2024Article
- Transgressive phenotypes from outbreeding between theMicrobiology spectrum · 2024Article
- Enhanced heterologous gene expression in Trichoderma reesei by promoting multicopy integration.Applied microbiology and biotechnology · 2024Article
- The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei.Scientific reports · 2024Article
- The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei.Communications biology · 2024Article
- Promoters and Synthetic Promoters in Trichoderma reesei.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30.BMC microbiology · 2023Article
- Alleviating vacuolar transport improves cellulase production in Trichoderma reesei.Applied microbiology and biotechnology · 2023Article
- Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi.Applied microbiology and biotechnology · 2022Article
- CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars.Biotechnology for biofuels · 2021Article
- Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei.Scientific reports · 2021Article
- The F-box protein geneProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Comparative genomics reveals a core gene toolbox for lifestyle transitions in Hypocreales fungi.Environmental microbiology · 2021Article
- Genetic Transformation of Trichoderma spp.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.BMC genomics · 2020Article
- Engineering ofBiotechnology for biofuels · 2020Article
- Whole Genome Sequencing Reveals Major Deletions in the Genome of M7, a Gamma Ray-Induced Mutant ofFrontiers in microbiology · 2020Article
28 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trichoderma reesei (teleomorph Hypocrea jecorina) is the main industrial source of cellulases and hemicellulases harnessed for the hydrolysis of biomass to simple sugars, which can then be converted to biofuels such as ethanol and other chemicals. The highly productive strains in use today were generated by classical mutagenesis. To learn how cellulase production was improved by these techniques, we performed massively parallel sequencing to identify mutations in the genomes of two hyperproducing strains (NG14, and its direct improved descendant, RUT C30). We detected a surprisingly high number of mutagenic events: 223 single nucleotides variants, 15 small deletions or insertions, and 18 larger deletions, leading to the loss of more than 100 kb of genomic DNA. From these events, we report previously undocumented non-synonymous mutations in 43 genes that are mainly involved in nuclear transport, mRNA stability, transcription, secretion/vacuolar targeting, and metabolism. This homogeneity of functional categories suggests that multiple changes are necessary to improve cellulase production and not simply a few clear-cut mutagenic events. Phenotype microarrays show that some of these mutations result in strong changes in the carbon assimilation pattern of the two mutants with respect to the wild-type strain QM6a. Our analysis provides genome-wide insights into the changes induced by classical mutagenesis in a filamentous fungus and suggests areas for the generation of enhanced T. reesei strains for industrial applications such as biofuel production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.