ArticleProceedings of the National Academy of Sciences of the United States of America2009
Hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Methanosarcina barkeri.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 93 citations in OpenAlex.
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- Pyruvate-dependent growth ofJournal of bacteriology · 2024Article
- Electron transport chains as a window into the earliest stages of evolution.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genetic and Physiological Probing of Cytoplasmic Bypasses for the Energy-Converting Methyltransferase Mtr in Methanosarcina acetivorans.Applied and environmental microbiology · 2023Article
- Integration of text mining and biological network analysis: Identification of essential genes in sulfate-reducing bacteria.Frontiers in microbiology · 2023Article
- Diversity of gut methanogens and functional enzymes associated with methane metabolism in smallholder dairy cattle.Archives of microbiology · 2022Article
- Insights into the biotechnology potential ofFrontiers in microbiology · 2022Review
- Cofactor F420: an expanded view of its distribution, biosynthesis and roles in bacteria and archaea.FEMS microbiology reviews · 2021Article
- Correlation of Key Physiological Properties ofApplied and environmental microbiology · 2021Article
- Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium.AMB Express · 2021Article
- Putative Extracellular Electron Transfer in Methanogenic Archaea.Frontiers in microbiology · 2021Review
- Efficient Photoelectron Capture by Ni Decoration in Methanosarcina barkeri-CdS Biohybrids for Enhanced Photocatalytic COiScience · 2020Article
- Different Interspecies Electron Transfer Patterns during Mesophilic and Thermophilic Syntrophic Propionate Degradation in Chemostats.Microbial ecology · 2020Article
- Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMicrobiology and molecular biology reviews : MMBR · 2019Review
- A Membrane-Bound Cytochrome EnablesmBio · 2019Article
- Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer.Journal of the Royal Society, Interface · 2019Article
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4 authors at 2 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Methanogens use an unusual energy-conserving electron transport chain that involves reduction of a limited number of electron acceptors to methane gas. Previous biochemical studies suggested that the proton-pumping F(420)H(2) dehydrogenase (Fpo) plays a crucial role in this process during growth on methanol. However, Methanosarcina barkeri Delta fpo mutants constructed in this study display no measurable phenotype on this substrate, indicating that Fpo plays a minor role, if any. In contrast, Delta frh mutants lacking the cytoplasmic F(420)-reducing hydrogenase (Frh) are severely affected in their ability to grow and make methane from methanol, and double Delta fpo/Delta frh mutants are completely unable to use this substrate. These data suggest that the preferred electron transport chain involves production of hydrogen gas in the cytoplasm, which then diffuses out of the cell, where it is reoxidized with transfer of electrons into the energy-conserving electron transport chain. This hydrogen-cycling metabolism leads directly to production of a proton motive force that can be used by the cell for ATP synthesis. Nevertheless, M. barkeri does have the flexibility to use the Fpo-dependent electron transport chain when needed, as shown by the poor growth of the Delta frh mutant. Our data suggest that the rapid enzymatic turnover of hydrogenases may allow a competitive advantage via faster growth rates in this freshwater organism. The mutant analysis also confirms the proposed role of Frh in growth on hydrogen/carbon dioxide and suggests that either Frh or Fpo is needed for aceticlastic growth of M. barkeri.
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