ArticleProceedings of the National Academy of Sciences of the United States of America2009
Phosphorylation of WASp is a key regulator of activity and stability in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
- Glycogen synthase kinase 3 inhibition controlsiScience · 2024Article
- Article
- Constitutive activation of WASp in X-linked neutropenia renders neutrophils hyperactive.The Journal of clinical investigation · 2018Article
- Wiskott-Aldrich syndrome protein: Emerging mechanisms in immunity.European journal of immunology · 2017Review
- Cytoskeletal control of B cell responses to antigens.Nature reviews. Immunology · 2017Review
- NPM-ALK phosphorylates WASp Y102 and contributes to oncogenesis of anaplastic large cell lymphoma.Oncogene · 2017Article
- Central role of PI3K-SYK interaction in fibrinogen-induced lamellipodia and filopodia formation in platelets.FEBS open bio · 2016Article
- The contribution of mouse models to the understanding of constitutional thrombocytopenia.Haematologica · 2016Review
- WASH has a critical role in NK cell cytotoxicity through Lck-mediated phosphorylation.Cell death & disease · 2016Article
- WASp-dependent actin cytoskeleton stability at the dendritic cell immunological synapse is required for extensive, functional T cell contacts.Journal of leukocyte biology · 2016Article
- Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex.Nature communications · 2015Article
- Wide-scale quantitative phosphoproteomic analysis reveals that cold treatment of T cells closely mimics soluble antibody stimulation.Journal of proteome research · 2015Article
- Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.eLife · 2015Article
- Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages.Journal of cell science · 2015Article
- X-linked thrombocytopenia causing mutations in WASP (L46P and A47D) impair T cell chemotaxis.Journal of biomedical science · 2014Article
- The F-BAR protein PSTPIP1 controls extracellular matrix degradation and filopodia formation in macrophages.Blood · 2014Article
- The pivotal position of the actin cytoskeleton in the initiation and regulation of B cell receptor activation.Biochimica et biophysica acta · 2014Review
- T cell antigen receptor activation and actin cytoskeleton remodeling.Biochimica et biophysica acta · 2014Review
- A new genetically encoded single-chain biosensor for Cdc42 based on FRET, useful for live-cell imaging.PloS one · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 7 institutions in 3 countries.
Funding
Abstract
The Wiskott-Aldrich syndrome protein (WASp) is a key cytoskeletal regulator in hematopoietic cells. Covalent modification of a conserved tyrosine by phosphorylation has emerged as an important potential determinant of activity, although the physiological significance remains uncertain. In a murine knockin model, mutation resulting in inability to phosphorylate Y293 (Y293F) mimicked many features of complete WASp-deficiency. Although a phosphomimicking mutant Y293E conferred enhanced actin-polymerization, the cellular phenotype was similar due to functional dysregulation. Furthermore, steady-state levels of Y293E-WASp were markedly reduced compared to wild-type WASp and Y293F-WASp, although partially recoverable by treatment of cells with proteasome inhibitors. Consequently, tyrosine phosphorylation plays a critical role in normal activation of WASp in vivo, and is indispensible for multiple tasks including proliferation, phagocytosis, chemotaxis, and assembly of adhesion structures. Furthermore, it may target WASp for proteasome-mediated degradation, thereby providing a default mechanism for self-limiting stimulation of the Arp2/3 complex.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.