ArticleProceedings of the National Academy of Sciences of the United States of America2009
Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 69 citations in OpenAlex.
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- Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila.Scientific reports · 2019Article
- Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungusPeerJ · 2018Article
- RNAi technology: a new platform for crop pest control.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Review
- Predicted trans-acting siRNAs in the human brain.International journal of molecular sciences · 2015Article
- Antiviral responses of arthropod vectors: an update on recent advances.Virusdisease · 2014Review
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- Tick cell lines for study of Crimean-Congo hemorrhagic fever virus and other arboviruses.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2012Review
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- Evolution of animal Piwi-interacting RNAs and prokaryotic CRISPRs.Briefings in functional genomics · 2012Review
- Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes.Insect molecular biology · 2012Article
- Male germline control of transposable elements.Biology of reproduction · 2012Review
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- Constitutive expression of short hairpin RNA in vivo triggers buildup of mature hairpin molecules.Human gene therapy · 2011Article
- RNA interference and heterochromatin assembly.Cold Spring Harbor perspectives in biology · 2011Review
- Interplay between innate immunity and negative-strand RNA viruses: towards a rational model.Microbiology and molecular biology reviews : MMBR · 2011Review
- Massive production of small RNAs from a non-coding region of Cauliflower mosaic virus in plant defense and viral counter-defense.Nucleic acids research · 2011Article
- Is RNA-dependent RNA polymerase essential for transposon control?BMC systems biology · 2011Article
- RNAi effector diversity in nematodes.PLoS neglected tropical diseases · 2011Article
Corrections and comments
- Retraction · 2011-08-05Error in Analyses ·
- Retracted
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here, we show that recombinant Drosophila elp1 (D-elp1) produced in Sf9 cells or Escherichia coli, corresponding to the largest of the three subunits in the RNA polymerase II core elongator complex, has RNA-dependent RNA polymerase (RdRP) activity. D-elp1 is a noncanonical RdRP that can synthesize dsRNA from different ssRNA templates using either a primer-dependent or primer-independent initiation mechanism. Of the three core subunits, only D-elp1 depletion inhibits RNAi in S2 cells but does not affect micro RNA function. Furthermore, D-elp1 depletion results in increased steady state levels of representative transposon RNAs and a decrease in the corresponding transposon antisense transcripts and endo siRNAs. In contrast, although Dcr-2 depletion results in increased transposon RNA levels and a reduction in the corresponding endo siRNAs, there is no change in the transposon antisense RNA levels. In D-elp1 null third instar larvae transposon RNA levels are also increased and the corresponding transposon antisense RNAs are reduced. D-elp1 associates tightly with Dcr-2, similar to the Dicer-RdRP interaction observed in lower eukaryotes. These results identify an aspect of the RNAi pathway in Drosophila that suggest transposon derived endo siRNAs, critical for transposon suppression, are produced, in part, in a D-elp1 dependent step that converts transposon RNA into dsRNA that is subsequently processed by Dcr-2. The generality of this mechanism in genome defense and RNA silencing in higher eukaryotes is suggested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.