ArticleProceedings of the National Academy of Sciences of the United States of America2009
In vivo far-red luminescence imaging of a biomarker based on BRET from Cypridina bioluminescence to an organic dye.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Quantitative Analysis of Bioluminescence Optical Signal.Biosensors · 2023Review
- In Vivo Imaging of Oxidative and Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Host-Dependent Producibility of RecombinantFrontiers in bioengineering and biotechnology · 2022Article
- Development of a miRNA-controlled dual-sensing system and its application for targeting miR-21 signaling in tumorigenesis.Experimental & molecular medicine · 2020Article
- BRET based dual-colour (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate.RSC advances · 2019Article
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules.Biosensors · 2019Review
- Leveraging Engineering of Indocyanine Green-Encapsulated Polymeric Nanocomposites for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2018Review
- The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis.Journal of controlled release : official journal of the Controlled Release Society · 2017Review
- A novel injectable BRET-based in vivo imaging probe for detecting the activity of hypoxia-inducible factor regulated by the ubiquitin-proteasome system.Scientific reports · 2016Article
- The Expanding Toolbox of In Vivo Bioluminescent Imaging.Frontiers in oncology · 2016Review
- A Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms.Molecular pharmacology · 2015Review
- A step beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL).Journal of visualized experiments : JoVE · 2014Article
- Article
- In vivo excitation of nanoparticles using luminescent bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Image guided biodistribution and pharmacokinetic studies of theranostics.Theranostics · 2012Review
- Bioluminescence imaging: progress and applications.Trends in biotechnology · 2011Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We aimed to develop a far-red luminescence imaging technology for visualization of disease specific antigens on cell surfaces in a living body. First, we conjugated a far-red fluorescent indocyanine derivative to biotinylated Cypridina luciferase. This conjugate produced a bimodal spectrum that has long-wavelength bioluminescence emission in the far-red region as a result of bioluminescence resonance energy transfer. To generate a far-red luminescent probe with targeting and imaging capabilities of tumors, we then linked this conjugate to an anti-human Dlk-1 monoclonal antibody via the biotin-avidin interaction. This far-red luminescent probe enabled us to obtain high-resolution microscopic images of live, Dlk-1-expressing Huh-7 cells without an external light source, and to monitor the accumulation of this probe in tumor-bearing mice. Thus this far-red luminescent probe is a convenient analytical tool for the evaluations of monoclonal antibody localization in a living body.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.