ArticleProceedings of the National Academy of Sciences of the United States of America2009
A strategy for blood biomarker amplification and localization using ultrasound.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Shock-scattering micro-histotripsy-aided fine needle aspiration for enhanced biomarker profiling and cytopathology.Nature communications · 2026Article
- Article
- Sonobiopsy for sensitive and spatially targeted molecular diagnosis through integrating ultrasound with liquid biopsy.NPJ acoustics · 2025Review
- Dynamic changes in extracellular vesicle-associated miRNAs elicited by ultrasound in inflammatory bowel disease patients.Scientific reports · 2024Article
- Barrier-breaking effects of ultrasonic cavitation for drug delivery and biomarker release.Ultrasonics sonochemistry · 2023Article
- Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles.ACS nano · 2022Review
- A Review of Imaging Methods to Assess Ultrasound-Mediated Ablation.BME frontiers · 2022Article
- Ultrasound-Mediated Drug Delivery: Sonoporation Mechanisms, Biophysics, and Critical Factors.BME frontiers · 2022Review
- Biomarkers and focused ultrasound: the future of liquid biopsy for brain tumor patients.Journal of neuro-oncology · 2022Review
- Synergies between therapeutic ultrasound, gene therapy and immunotherapy in cancer treatment.Advanced drug delivery reviews · 2021Review
- Synthetic biomarkers: a twenty-first century path to early cancer detection.Nature reviews. Cancer · 2021Review
- Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model.Scientific reports · 2020Article
- Applications of Focused Ultrasound in Cerebrovascular Diseases and Brain Tumors.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2019Review
- Focused Ultrasound-enabled Brain Tumor Liquid Biopsy.Scientific reports · 2018Article
- Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence.PloS one · 2018Article
- Ultrasound and microbubble induced release from intracellular compartments.BMC biotechnology · 2017Article
- Passive Acoustic Mapping with the Angular Spectrum Method.IEEE transactions on medical imaging · 2017Article
- Article
- Mathematical framework for activity-based cancer biomarkers.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Detecting cancers through tumor-activatable minicircles that lead to a detectable blood biomarker.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Blood biomarkers have significant potential applications in early detection and management of various diseases, including cancer. Most biomarkers are present in low concentrations in blood and are difficult to discriminate from noise. Furthermore, blood measurements of a biomarker do not provide information about the location(s) where it is produced. We hypothesize a previously undescribed strategy to increase the concentration of biomarkers in blood as well as localize the source of biomarker signal using ultrasound energy directly applied to tumor cells. We test and validate our hypothesis in cell culture experiments and mouse tumor xenograft models using the human colon cancer cell line LS174T, while measuring the biomarker carcinoembryonic antigen (CEA) before and after the use of ultrasound to liberate the biomarker from the tumor cells. The results demonstrate that the application of low-frequency ultrasound to tumor cells causes a significant release of tumor biomarker, which can be measured in the blood. Furthermore, we establish that this release is specific to the direct application of the ultrasound to the tumor, enabling a method for localization of biomarker production. This work shows that it is possible to use ultrasound to amplify and localize the source of CEA levels in blood of tumor-bearing mice and will allow for a previously undescribed way to determine the presence and localization of disease more accurately using a relatively simple and noninvasive strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.