ArticleProceedings of the National Academy of Sciences of the United States of America2009
Orthogonal analytical approaches to detect potential contaminants in heparin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 92 citations in OpenAlex.
- Approaches to Assure Similarity between Pharmaceutical Heparins from Two Different Manufacturers.Pharmaceutics · 2023Article
- Pentosan Polysulfate Inhibits Attachment and Infection by SARS-CoV-2 In Vitro: Insights into Structural Requirements for Binding.Thrombosis and haemostasis · 2022Article
- Review
- Tools for the Quality Control of Pharmaceutical Heparin.Medicina (Kaunas, Lithuania) · 2019Review
- Crude Heparin Preparations Unveil the Presence of Structurally Diverse Oversulfated Contaminants.Molecules (Basel, Switzerland) · 2019Article
- Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.Molecules (Basel, Switzerland) · 2017Article
- A Fluorescent Probe for Glycosaminoglycans Applied to the Detection of Dermatan Sulfate by a Mix-and-Read Assay.Molecules (Basel, Switzerland) · 2017Article
- Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.Analytical chemistry · 2016Article
- Discovery of an intrinsic tenase complex inhibitor: Pure nonasaccharide from fucosylated glycosaminoglycan.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Polymer - Nanoparticle Assemblies for Array Based Sensing.Current organic chemistry · 2015Article
- Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells.Cytotechnology · 2015Article
- Structure and activity of a new low-molecular-weight heparin produced by enzymatic ultrafiltration.Journal of pharmaceutical sciences · 2014Article
- Method to detect contaminants in heparin using radical depolymerization and liquid chromatography-mass spectrometry.Analytical chemistry · 2014Article
- Structural characterization of pharmaceutical heparins prepared from different animal tissues.Journal of pharmaceutical sciences · 2013Article
- Linear polyalkylamines as fingerprinting agents in capillary electrophoresis of low-molecular-weight heparins and glycosaminoglycans.Electrophoresis · 2011Article
- Structural characterization of heparins from different commercial sources.Analytical and bioanalytical chemistry · 2011Article
- Competitive inhibition of heparinase by persulfonated glycosaminoglycans: a tool to detect heparin contamination.Analytical chemistry · 2011Article
- Controlled Photochemical Depolymerization of K5 Heparosan, a Bioengineered Heparin Precursor.Carbohydrate polymers · 2011Article
- Article
- Analysis of E. coli K5 capsular polysaccharide heparosan.Analytical and bioanalytical chemistry · 2011Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Heparin is a widely used anticoagulant and antithrombotic agent. Recently, a contaminant, oversulfated chondroitin sulfate (OSCS), was discovered within heparin preparations. The presence of OSCS within heparin likely led to clinical manifestations, most prevalently, hypotension and abdominal pain leading to the deaths of several dozens of patients. Given the biological effects of OSCS, one continuing item of concern is the ability for existing methods to identify other persulfonated polysaccharide compounds that would also have anticoagulant activity and would likely elicit a similar activation of the contact system. To complete a more extensive analysis of the ability for NMR and capillary electrophoresis (CE) to capture a broader array of potential contaminants within heparin, we completed a systematic study of NMR, both mono- and bidimensional, and CE to detect both various components of sidestream heparin and their persulfonated derivatives. We show that given the complexity of heparin samples, and the requirement to ensure their purity and safety, use of orthogonal analytical techniques is effective at detecting an array of potential contaminants that could be present.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.