ArticleProceedings of the National Academy of Sciences of the United States of America2009
Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Conserved serum protein biomarkers associated with growing early colorectal adenomas.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression.Oncotarget · 2016Article
- The utility of Apc-mutant rats in modeling human colon cancer.Disease models & mechanisms · 2014Review
- The concentrations of EGFR, LRG1, ITIH4, and F5 in serum correlate with the number of colonic adenomas in ApcPirc/+ rats.Cancer prevention research (Philadelphia, Pa.) · 2014Article
- Candidate serum biomarkers for early intestinal cancer using 15N metabolic labeling and quantitative proteomics in the ApcMin/+ mouse.Journal of proteome research · 2013Article
- Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Carboxylesterases 1 and 2 hydrolyze phospho-nonsteroidal anti-inflammatory drugs: relevance to their pharmacological activity.The Journal of pharmacology and experimental therapeutics · 2012Article
- Quantification of human uridine-diphosphate glucuronosyl transferase 1A isoforms in liver, intestine, and kidney using nanobore liquid chromatography-tandem mass spectrometry.Analytical chemistry · 2012Article
- Proteins and an inflammatory network expressed in colon tumors.Journal of proteome research · 2011Article
- In planta changes in protein phosphorylation induced by the plant hormone abscisic acid.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The conserved bromo-adjacent homology domain of yeast Orc1 functions in the selection of DNA replication origins within chromatin.Genes & development · 2010Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
The unique biology of a neoplasm is reflected by its distinct molecular profile compared with normal tissue. To understand tumor development better, we have undertaken a quantitative proteomic search for abnormally expressed proteins in colonic tumors from Apc(Min/+) (Min) mice. By raising pairs of Min and wild-type mice on diets derived from natural-abundance or (15)N-labeled algae, we used metabolic labeling to compare protein levels in colonic tumor versus normal tissue. Because metabolic labeling allows internal control throughout sample preparation and analysis, technical error is minimized as compared with in vitro labeling. Several proteins displayed altered expression, and a subset was validated via stable isotopic dilution using synthetic peptide standards. We also compared gene and protein expression among tumor and nontumor tissue, revealing limited correlation. This divergence was especially pronounced for species showing biological change, highlighting the complementary perspectives provided by transcriptomics and proteomics. Our work demonstrates the power of metabolic labeling combined with stable isotopic dilution as an integrated strategy for the identification and validation of differentially expressed proteins using rodent models of human disease.
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