ArticleBiophysical journal2009
Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 110 citations in OpenAlex.
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- Mechanical cues enhance chondrocyte function: Insights from mechanoreception, regulation, and biological responses.Mechanobiology in medicine · 2025Review
- Compression-dependent microtubule reinforcement enables cells to navigate confined environments.Nature cell biology · 2024Article
- Mechanical models and measurement methods of solid stress in tumors.Applied microbiology and biotechnology · 2024Review
- Membrane stretch as the mechanism of activation of PIEZO1 ion channels in chondrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Mechanoreception inCells · 2023Article
- Biomechanics of Chondrocytes and Chondrons in Healthy Conditions and Osteoarthritis: A Review of the Mechanical Characterisations at the Microscale.Biomedicines · 2023Review
- Stiffened fibre-like microenvironment based on patterned equidistant micropillars directs chondrocyte hypertrophy.Materials today. Bio · 2023Article
- Mechanical Regulation of Mitochondrial Dynamics and Function in a 3D-Engineered Liver Tumor Microenvironment.ACS biomaterials science & engineering · 2023Article
- The antimetastatic breast cancer activity of the viral protein-derived peptide vCPP2319 as revealed by cellular biomechanics.The FEBS journal · 2022Article
- Poroelastic osmoregulation of living cell volume.iScience · 2021Article
- B16 Melanoma Cancer Cells with Higher Metastatic Potential are More Deformable at a Whole-Cell Level.Cellular and molecular bioengineering · 2021Article
- Molecular cancer cell responses to solid compressive stress and interstitial fluid pressure.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Combination of chondrocytes and chondrons improves extracellular matrix production to promote the repairs of defective knee cartilage in rabbits.Journal of orthopaedic translation · 2021Article
- Hydrostatic pressure prevents chondrocyte differentiation through heterochromatin remodeling.Journal of cell science · 2021Article
- Aging, Osteo-Sarcopenia, and Musculoskeletal Mechano-Transduction.Frontiers in rehabilitation sciences · 2021Article
- Mechanosensation in traumatic brain injury.Neurobiology of disease · 2021Review
- A NanoFE simulation-based surrogate machine learning model to predict mechanical functionality of protein networks from live confocal imaging.Computational and structural biotechnology journal · 2020Article
- On-chip surface acoustic wave and micropipette aspiration techniques to assess cell elastic properties.Biomicrofluidics · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The cytoskeleton is known to play an important role in the biomechanical nature and structure of cells, but its particular function in compressive characteristics has not yet been fully examined. This study focused on the contribution of the main three cytoskeletal elements to the bulk compressive stiffness (as measured by the compressive modulus), volumetric or apparent compressibility changes (as further indicated by apparent Poisson's ratio), and recovery behavior of individual chondrocytes. Before mechanical testing, cytochalasin D, acrylamide, or colchicine was used to disrupt actin microfilaments, intermediate filaments, or microtubules, respectively. Cells were subjected to a range of compressive strains and allowed to recover to equilibrium. Analysis of the video recording for each mechanical event yielded relevant compressive properties and recovery characteristics related to the specific cytoskeletal disrupting agent and as a function of applied axial strain. Inhibition of actin microfilaments had the greatest effect on bulk compressive stiffness ( approximately 50% decrease compared to control). Meanwhile, intermediate filaments and microtubules were each found to play an integral role in either the diminution (compressibility) or retention (incompressibility) of original cell volume during compression. In addition, microtubule disruption had the largest effect on the "critical strain threshold" in cellular mechanical behavior (33% decrease compared to control), as well as the characteristic time for recovery ( approximately 100% increase compared to control). Elucidating the role of the cytoskeleton in the compressive biomechanical behavior of single cells is an important step toward understanding the basis of mechanotransduction and the etiology of cellular disease processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.