ArticleBiophysical journal2009
Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- JiSuJi, a virtual muscle for small animal simulations, accurately predicts force from naturalistic spike trains.bioRxiv : the preprint server for biology · 2025Article
- Ratiometric Imaging for Quantification of Elevated CaACS chemical neuroscience · 2025Article
- Excitation-contraction coupling in mammalian skeletal muscle: Blending old and last-decade research.Frontiers in physiology · 2022Review
- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- Insights Into Spinal Dorsal Horn Circuit Function and Dysfunction Using Optical Approaches.Frontiers in neural circuits · 2020Review
- Modeling and simulation of excitation- contraction coupling of fast-twitch skeletal muscle fibers.Technology and health care : official journal of the European Society for Engineering and Medicine · 2020Article
- Extraocular muscle function is impaired inThe Journal of general physiology · 2019Article
- A study of the mechanisms of excitation-contraction coupling in frog skeletal muscle based on measurements of [CaJournal of muscle research and cell motility · 2018Review
- The relationship between form and function throughout the history of excitation-contraction coupling.The Journal of general physiology · 2018Review
- Small CaThe Journal of general physiology · 2018Article
- Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates.eLife · 2017Article
- CNGC2 Is a Ca2+ Influx Channel That Prevents Accumulation of Apoplastic Ca2+ in the Leaf.Plant physiology · 2017Article
- Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles.The Journal of biological chemistry · 2016Article
- Modeling CaFrontiers in physiology · 2016Article
- Raptor ablation in skeletal muscle decreases Cav1.1 expression and affects the function of the excitation-contraction coupling supramolecular complex.The Biochemical journal · 2015Article
- Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach.Journal of muscle research and cell motility · 2014Article
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Article
- Enhanced dihydropyridine receptor calcium channel activity restores muscle strength in JP45/CASQ1 double knockout mice.Nature communications · 2013Article
- Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.Circulation research · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of the above indicators and, usually, furaptra (previously shown to rapidly track Delta[Ca(2+)]). In response to an action potential, the full duration at half-maximum of the indicator's fluorescence change (DeltaF) was found to be larger with OGB-5N, Fluo-5N, fura-5N, and Rhod-5N than with furaptra; thus, these indicators do not track Delta[Ca(2+)] with kinetic fidelity. In contrast, the DeltaF time course of Mag-fluo-4 was identical to furaptra's; thus, Mag-fluo-4 also yields reliable kinetic information about Delta[Ca(2+)]. Mag-fluo-4's DeltaF has a larger signal/noise ratio than furaptra's (for similar indicator concentrations), and should thus be more useful for tracking Delta[Ca(2+)] in small cell volumes. However, because the resting fluorescence of Mag-fluo-4 probably arises largely from indicator that is bound with Mg(2+), the amplitude of the Mag-fluo-4 signal, and its calibration in Delta[Ca(2+)] units, is likely to be more sensitive to variations in [Mg(2+)] than furaptra's.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.