ArticleAnalytical chemistry2009
Microfluidic chip for high efficiency electrophoretic analysis of segmented flow from a microdialysis probe and in vivo chemical monitoring.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 106 citations in OpenAlex.
- Microfluidic-based electrically driven particle manipulation techniques for biomedical applications.RSC advances · 2025Review
- Microdialysis coupled with droplet microfluidics and mass spectrometry for determination of neurotransmittersThe Analyst · 2024Article
- Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform.Lab on a chip · 2021Article
- Active Flow Control and Dynamic Analysis in Droplet Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Review
- In-Droplet Electrophoretic Separation and Enrichment of Biomolecules.Analytical chemistry · 2020Article
- Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis.Micromachines · 2020Review
- Portable Microfluidic Biosensing System for Real-Time Analysis of Microdialysate in Transplant Kidneys.Analytical chemistry · 2019Article
- Droplet sample introduction to microchip gel and zone electrophoresis for rapid analysis of protein-protein complexes and enzymatic reactions.Analytical and bioanalytical chemistry · 2019Article
- Monitoring biomolecule concentrations in tissue using a wearable droplet microfluidic-based sensor.Nature communications · 2019Article
- Review
- Microfabricated needle for hydrogen peroxide detection.RSC advances · 2019Article
- Use and Future Prospects of in Vivo Microdialysis for Epilepsy Studies.ACS chemical neuroscience · 2019Review
- In Vivo Chemical Monitoring at High Spatiotemporal Resolution Using Microfabricated Sampling Probes and Droplet-Based Microfluidics Coupled to Mass Spectrometry.Analytical chemistry · 2018Article
- Advancement of analytical modes in a multichannel, microfluidic droplet-based sample chopper employing phase-locked detection.Analytical methods : advancing methods and applications · 2018Article
- Microfabricated Probes for Studying Brain Chemistry: A Review.Chemphyschem : a European journal of chemical physics and physical chemistry · 2018Review
- Microfluidic Droplet Extraction by Hydrophilic Membrane.Micromachines · 2017Article
- Advances in capillary electrophoresis and the implications for drug discovery.Expert opinion on drug discovery · 2017Review
- Automatic Combination of Microfluidic Nanoliter-Scale Droplet Array with High-Speed Capillary Electrophoresis.Scientific reports · 2016Article
- Identification of sirtuin 5 inhibitors by ultrafast microchip electrophoresis using nanoliter volume samples.Analytical and bioanalytical chemistry · 2016Article
- Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides.Biochimica et biophysica acta · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
An effective method for in vivo chemical monitoring is to couple sampling probes, such as microdialysis, to online analytical methods. A limitation of this approach is that in vivo chemical dynamics may be distorted by flow and diffusion broadening during transfer from sampling probe to analytical system. Converting a homogeneous sample stream to segmented flow can prevent such broadening. We have developed a system for coupling segmented microdialysis flow with chip-based electrophoresis. In this system, the dialysis probe is integrated with a PDMS chip that merges dialysate with fluorogenic reagent and segments the flow into 8-10 nL plugs at 0.3-0.5 Hz separated by perfluorodecalin. The plugs flow to a glass chip where they are extracted to an aqueous stream and analyzed by electrophoresis with fluorescence detection. The novel extraction system connects the segmented flow to an electrophoresis sampling channel by a shallow and hydrophilic extraction bridge that removes the entire aqueous droplet from the oil stream. With this approach, temporal resolution was 35 s and independent of distance between sampling and analysis. Electrophoretic analysis produced separation with 223,000 +/- 21,000 theoretical plates, 4.4% RSD in peak height, and detection limits of 90-180 nM for six amino acids. This performance was made possible by three key elements: (1) reliable transfer of plug flow to a glass chip; (2) efficient extraction of aqueous plugs from segmented flow; (3) electrophoretic injection suitable for high efficiency separation with minimal dilution of sample. The system was used to detect rapid concentration changes evoked by infusing glutamate uptake inhibitor into the striatum of anesthetized rats. These results demonstrate the potential of incorporating segmented flow into separations-based sensing schemes for studying chemical dynamics in vivo with improved temporal resolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.