ArticleNature biotechnology2009
Sensitive multiplexed analysis of kinase activities and activity-based kinase identification.
Article in Nature biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed.
- Protocol to study direct (de)phosphorylation events on a proteome-wide scale using on-bead in vitro enzyme assays.STAR protocols · 2026Article
- LIMS-Kinase provides sensitive and generalizable label-freeCell reports. Physical science · 2023Article
- Pharmacological approaches to understanding protein kinase signaling networks.Frontiers in pharmacology · 2023Review
- Shining Light on Protein Kinase Biomarkers with Fluorescent Peptide Biosensors.Life (Basel, Switzerland) · 2022Review
- Interrogating Kinase-Substrate Relationships with Proximity Labeling and Phosphorylation Enrichment.Journal of proteome research · 2022Article
- Strategies for mass spectrometry-based phosphoproteomics using isobaric tagging.Expert review of proteomics · 2021Review
- Advances in quantitative high-throughput phosphoproteomics with sample multiplexing.Proteomics · 2021Review
- Drug Discovery in Liver Disease Using Kinome Profiling.International journal of molecular sciences · 2021Review
- Versatile small molecule kinase assay through real-time, ratiometric fluorescence changes based on a pyrene-DPA-ZnRSC advances · 2021Article
- Multiplex enzyme activity imaging by MALDI-IMS of substrate library conversions.Scientific reports · 2020Article
- Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites.Nature communications · 2020Article
- An ultrasensitive fiveplex activity assay for cellular kinases.Scientific reports · 2019Article
- Large-scale Discovery of Substrates of the Human Kinome.Scientific reports · 2019Article
- Mapping phospho-catalytic dependencies of therapy-resistant tumours reveals actionable vulnerabilities.Nature cell biology · 2019Article
- Assays for tyrosine phosphorylation in human cells.Methods in enzymology · 2019Article
- Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.Journal of proteomics · 2018Review
- Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library.Biochemistry · 2018Article
- Multiplex Substrate Profiling by Mass Spectrometry for Kinases as a Method for Revealing Quantitative Substrate Motifs.Analytical chemistry · 2017Article
- Recent advances in methods to assess the activity of the kinome.F1000Research · 2017Review
- Studying Cellular Signal Transduction with OMIC Technologies.Journal of molecular biology · 2015Review
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16 authors.
Funding
Abstract
Constitutive activation of one or more kinase signaling pathways is a hallmark of many cancers. Here we extend the previously described mass spectrometry-based KAYAK approach by monitoring kinase activities from multiple signaling pathways simultaneously. This improved single-reaction strategy, which quantifies the phosphorylation of 90 synthetic peptides in a single mass spectrometry run, is compatible with nanogram to microgram amounts of cell lysate. Furthermore, the approach enhances kinase monospecificity through substrate competition effects, faithfully reporting the signatures of many signaling pathways after mitogen stimulation or of basal pathway activation differences across a panel of well-studied cancer cell lines. Hierarchical clustering of activities from related experiments groups peptides phosphorylated by similar kinases together and, when combined with pathway alteration using pharmacological inhibitors, distinguishes underlying differences in potency, off-target effects and genetic backgrounds. Finally, we introduce a strategy to identify the kinase, and even associated protein complex members, responsible for phosphorylation events of interest.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.