Evidence map›Paper›PMID 19801719›Full record

ArticleCurrent issues in molecular biology2010

Green technologies for room temperature nucleic acid storage.

Eunice Wan, Matthew Akana, Jennifer Pons, Justin Chen, Stacy Musone, Pui-Yan Kwok, Wilson Liao

Abstract read
In one paragraph

Article in Current issues in molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
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  14. Review
  15. An efficient method for long-term room temperature storage of RNA.European journal of human genetics : EJHG · 2014
    Article
  16. Article
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Eunice WanCardiovascular Research Institute, University of California, San Francisco, 94143, USA.
Matthew Akana
Jennifer Pons
Justin Chen
Stacy Musone
Pui-Yan Kwok
Wilson Liao

Funding

TECHNOLOGIES FOR DIALLELIC MARKER DISCOVERY AND TESTINGR01HG001720 · NHGRI · WASHINGTON UNIVERSITY · PI KWOK, PUI-YAN · 1997 to 2007
$9.6M
UCSF Dermatology Training GrantT32AR007175 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Wilson Liao, Michael David Rosenblum · 1986 to 2026
$6.1M
Genetic Analysis of the TNF Pathway in PsoriasisK08AR057763 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIAO, WILSON · 2010 to 2014
$599k
NHGRI NIH HHS R01 HG001720NHGRI NIH HHS R01-HG01720NIAMS NIH HHS K08 AR057763NIAMS NIH HHS T32 AR007175
6 · The paper itself

Abstract

Maintaining the long-term integrity of nucleic acids in the laboratory has traditionally required the use of freezers. However, novel nucleic acid stabilization technologies may allow for the storage of DNA and RNA at room temperature in a cost-effective, environmentally friendly manner. In this study, we evaluated two novel products for room temperature DNA storage: Biomatrica's DNA SampleMatrix technology and GenVault's GenTegra DNA technology. We compared the integrity and quality of DNA stored using these products against DNA stored in a -20 C freezer by performing downstream testing with short range PCR, long range PCR, DNA sequencing, and SNP microarrays. In addition, we tested Biomatrica's RNAstable product for its ability to preserve RNA at room temperature for use in a quantitative reverse transcription PCR assay.

Indexed as

Nucleic AcidsTemperatureDNAFreezingGenome, HumanGreen Chemistry TechnologyHumansOligonucleotide Array Sequence AnalysisPreservation, BiologicalReverse Transcriptase Polymerase Chain ReactionRNASequence Analysis, DNASpecimen HandlingDNANucleic AcidsRNA

Identifiers

PMID19801719
PMCPMC4099058

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.