ArticleThe Journal of biological chemistry2009
Characterization of human cone phosphodiesterase-6 ectopically expressed in Xenopus laevis rods.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 27 citations in OpenAlex.
- Photoreceptor phosphodiesterase (PDE6): activation and inactivation mechanisms during visual transduction in rods and cones.Pflugers Archiv : European journal of physiology · 2021Review
- Diminished Cone Sensitivity in cpfl3 Mice Is Caused by Defective Transducin Signaling.Investigative ophthalmology & visual science · 2020Article
- Raftophilic rhodopsin-clusters offer stochastic platforms for G protein signalling in retinal discs.Communications biology · 2019Article
- Retinal tissue preparation for high-resolution live imaging of photoreceptors expressing multiple transgenes.MethodsX · 2018Article
- Mechanisms of mutant PDE6 proteins underlying retinal diseases.Cellular signalling · 2017Article
- Molecular basis for photoreceptor outer segment architecture.Progress in retinal and eye research · 2016Review
- Aryl Hydrocarbon Receptor-interacting Protein-like 1 Is an Obligate Chaperone of Phosphodiesterase 6 and Is Assisted by the γ-Subunit of Its Client.The Journal of biological chemistry · 2016Article
- Targeted ablation of the Pde6h gene in mice reveals cross-species differences in cone and rod phototransduction protein isoform inventory.The Journal of biological chemistry · 2015Article
- Distinct patterns of compartmentalization and proteolytic stability of PDE6C mutants linked to achromatopsia.Molecular and cellular neurosciences · 2015Article
- Activation and quenching of the phototransduction cascade in retinal cones as inferred from electrophysiology and mathematical modeling.Molecular vision · 2015Article
- Submembrane assembly and renewal of rod photoreceptor cGMP-gated channel: insight into the actin-dependent process of outer segment morphogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- The GAFa domain of phosphodiesterase-6 contains a rod outer segment localization signal.Journal of neurochemistry · 2014Article
- Article
- Organization of cGMP sensing structures on the rod photoreceptor outer segment plasma membrane.Channels (Austin, Tex.) · 2014Article
- Cone phosphodiesterase-6α' restores rod function and confers distinct physiological properties in the rod phosphodiesterase-6β-deficient rd10 mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Identification of amino acid residues responsible for the selectivity of tadalafil binding to two closely related phosphodiesterases, PDE5 and PDE6.The Journal of biological chemistry · 2012Article
- Speed, sensitivity, and stability of the light response in rod and cone photoreceptors: facts and models.Progress in retinal and eye research · 2012Review
- Atypical retinal degeneration 3 in mice is caused by defective PDE6B pre-mRNA splicing.Vision research · 2012Article
- Speed, adaptation, and stability of the response to light in cone photoreceptors: the functional role of Ca-dependent modulation of ligand sensitivity in cGMP-gated ion channels.The Journal of general physiology · 2012Article
- Decreased catalytic activity and altered activation properties of PDE6C mutants associated with autosomal recessive achromatopsia.Human molecular genetics · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
PDE6 (phosphodiesterase-6) is the effector molecule in the vertebrate phototransduction cascade. Progress in understanding the structure and function of PDE6 has been hindered by lack of an expression system of the enzyme. Here we report ectopic expression and analysis of compartmentalization and membrane dynamics of the enhanced green fluorescent protein (EGFP) fusion protein of human cone PDE6C in rods of transgenic Xenopus laevis. EGFP-PDE6C is correctly targeted to the rod outer segments in transgenic Xenopus, where it displayed a characteristic striated pattern of EGFP fluorescence. Immunofluorescence labeling indicated significant and light-independent co-localization of EGFP-PDE6C with the disc rim marker peripherin-2 and endogenous frog PDE6. The diffusion of EGFP-PDE6C on disc membranes investigated with fluorescence recovery after photobleaching was markedly slower than theoretically predicted. The enzymatic characteristics of immunoprecipitated recombinant PDE6C were similar to known properties of the native bovine PDE6C. PDE6C was potently inhibited by the cone- and rod-specific PDE6 gamma-subunits. Thus, transgenic Xenopus laevis is a unique expression system for PDE6 well suited for analysis of the mechanisms of visual diseases linked to PDE6 mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.