ArticleThe Journal of biological chemistry2009
An RNA recognition motif mediates the nucleocytoplasmic transport of a trypanosome RNA-binding protein.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Modulation of Virulence Factors duringPathogens (Basel, Switzerland) · 2022Review
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Translational repression by an RNA-binding protein promotes differentiation to infective forms in Trypanosoma cruzi.PLoS pathogens · 2018Article
- Nuclear distribution of the Trypanosoma cruzi RNA Pol I subunit RPA31 during growth and metacyclogenesis, and characterization of its nuclear localization signal.Parasitology research · 2018Article
- Cytoplasmic poly(A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells.The Journal of biological chemistry · 2017Article
- Nuclear Compartmentalization Contributes to Stage-Specific Gene Expression Control inFrontiers in cell and developmental biology · 2017Article
- Comparative genomics of drug resistance in Trypanosoma brucei rhodesiense.Cellular and molecular life sciences : CMLS · 2016Article
- Regulation of RNA binding proteins in trypanosomatid protozoan parasites.World journal of biological chemistry · 2016Review
- Interactions between RNA-binding proteins and P32 homologues in trypanosomes and human cells.Current genetics · 2016Article
- Differential Subcellular Localization of Leishmania Alba-Domain Proteins throughout the Parasite Development.PloS one · 2015Article
- Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast.BMC microbiology · 2014Article
- Free mRNA in excess upon polysome dissociation is a scaffold for protein multimerization to form stress granules.Nucleic acids research · 2014Article
- The mRNAs associated to a zinc finger protein from Trypanosoma cruzi shift during stress conditions.RNA biology · 2014Article
- Article
- Unravelling the Neospora caninum secretome through the secreted fraction (ESA) and quantification of the discharged tachyzoite using high-resolution mass spectrometry-based proteomics.Parasites & vectors · 2013Article
- The RRM domain of human fused in sarcoma protein reveals a non-canonical nucleic acid binding site.Biochimica et biophysica acta · 2013Article
- mRNA localization mechanisms in Trypanosoma cruzi.PloS one · 2013Article
- Article
- Proteomics on the rims: insights into the biology of the nuclear envelope and flagellar pocket of trypanosomes.Parasitology · 2012Review
- Alterations in DRBD3 ribonucleoprotein complexes in response to stress in Trypanosoma brucei.PloS one · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA-binding proteins (RBPs) and RNA metabolism are considered to be important for modulating gene expression in trypanosomes, because these protozoan parasites mainly rely on post-transcriptional mechanisms to regulate protein levels. Previously, we have identified TcUBP1, a single RNA recognition motif (RRM)-type RBP from Trypanosoma cruzi. TcUBP1 is a cytoplasmic protein with roles in stabilization/degradation of mRNAs and in the protection of transcripts through their recruitment into cytoplasmic granules. We now show that TcUBP1, and the closely related protein TcUBP2, can be found in small amounts in the nucleus under normal conditions, and are able to accumulate in the nucleus under arsenite stress. The kinetics of nuclear accumulation, and export to the cytoplasm, are consistent with the shuttling of TcUBP1 between the nucleus and the cytoplasm. The sequence required for TcUBP1 nuclear accumulation was narrowed to the RRM, and point mutations affecting RNA binding abolished nuclear import. This RRM was also shown to be efficiently exported from the nucleus in unstressed parasites, a property that relied on the binding to RNA. TcUBP1 nuclear accumulation was dependent on active transcription, and colocalized with transcripts in the nucleus, suggesting nuclear binding of the mRNA. We propose that TcUBP1 could be linking the mRNA metabolism at both sides of the nuclear pore complex, using the RRM as a nuclear localization signal, and being exported as a cargo on mRNA.
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