ArticleJournal of immunology (Baltimore, Md. : 1950)2009
PU.1 regulates TCR expression by modulating GATA-3 activity.
Article in Journal of immunology (Baltimore, Md. : 1950), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 67 citations in OpenAlex.
- Fetal lymphocyte progenitors efficiently generate innate-like B cells but make a more limited contribution to the production of conventional lymphocytes.Experimental hematology · 2025Review
- Transient PU.1 low fetal progenitors generate lymphoid progeny that contribute to adult immunity.Life science alliance · 2024Article
- STAT3 Signaling Heterogeneity Underlies Cytokine-Expressing Fate in Th17 Cultures.ImmunoHorizons · 2023Article
- T helper 2 cells in asthma.The Journal of experimental medicine · 2023Review
- PU.1-c-Jun interaction is crucial for PU.1 function in myeloid development.Communications biology · 2022Article
- Realization of the T Lineage Program Involves GATA-3 Induction of Bcl11b and Repression of Cdkn2b Expression.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis.BMC immunology · 2022Article
- Expression profile of PU.1 in CD4Clinical and experimental medicine · 2021Article
- Cigarette smoke induction of S100A9 contributes to chronic obstructive pulmonary disease.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Aberrant MicroRNAomics in Pulmonary Complications: Implications in Lung Health and Diseases.Molecular therapy. Nucleic acids · 2019Review
- A conserved enhancer regulates Il9 expression in multiple lineages.Nature communications · 2018Article
- Transcriptional Control of Th9 Cells: Role of Foxo1 in Interleukin-9 Induction.Frontiers in immunology · 2018Review
- Effects of allergic stimulation and glucocorticoids on miR-155 in CD4American journal of clinical and experimental immunology · 2018Article
- Transcription factor Foxo1 is essential for IL-9 induction in T helper cells.Nature communications · 2017Article
- IL-9 and Th9 cells in health and diseases-From tolerance to immunopathology.Cytokine & growth factor reviews · 2017Review
- Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients.BMC immunology · 2017Article
- The transcription factor network in Th9 cells.Seminars in immunopathology · 2017Review
- Th9 and other IL-9-producing cells in allergic asthma.Seminars in immunopathology · 2017Review
- The ETS Family Transcription Factors Etv5 and PU.1 Function in Parallel To Promote Th9 Cell Development.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- An Lck-cre transgene accelerates autoantibody production and lupus development in (NZB × NZW)F1 mice.Lupus · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Ets transcription factor PU.1 is a master regulator for the development of multiple lineages during hematopoiesis. The expression pattern of PU.1 is dynamically regulated during early T lineage development in the thymus. We previously revealed that PU.1 delineates heterogeneity of effector Th2 populations. In this study, we further define the function of PU.1 on the Th2 phenotype using mice that specifically lack PU.1 in T cells using an lck-Cre transgene with a conditional Sfpi1 allele (Sfpi1(lck-/-)). Although deletion of PU.1 by the lck-Cre transgene does not affect T cell development, Sfpi1(lck-/-) T cells have a lower activation threshold than wild-type T cells. When TCR engagement is limiting, Sfpi1(lck-/-) T cells cultured in Th2 polarizing conditions secrete higher levels of Th2 cytokines and have greater cytokine homogeneity than wild-type cells. We show that PU.1 modulates the levels of TCR expression in CD4(+) T cells by regulating the DNA-binding activity of GATA-3 and limiting GATA-3 regulation of TCR gene expression. GATA-3-dependent regulation of TCR expression is also observed in Th1 and Th2 cells. In CD4(+) T cells, PU.1 expression segregates into subpopulations of cells that have lower levels of surface TCR, suggesting that PU.1 contributes to the heterogeneity of TCR expression. Thus, we have identified a mechanism whereby increased GATA-3 function in the absence of the antagonizing activity of PU.1 leads to increased TCR expression, a reduced activation threshold, and increased homogeneity in Th2 populations.
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