Evidence map›Paper›PMID 19801463›Full record

ArticleApplied and environmental microbiology2009

Development and application of quantitative-PCR tools for subgroups of the Roseobacter clade.

Alison Buchan, Mary Hadden, Marcelino T Suzuki

Abstract readEvaluation Study
In one paragraph

Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alison BuchanDepartment of Microbiology, University of Tennessee, Knoxville, TN 37996, USA. abuchan@utk.edu
Mary Hadden
Marcelino T Suzuki

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.

Indexed as

Bacteriological TechniquesBenzothiazolesBiodiversityDiaminesDNA, BacterialDNA, Ribosomal SpacerEnvironmental MicrobiologyFluorescent DyesMolecular Sequence DataOrganic ChemicalsPhylogenyPolymerase Chain ReactionQuinolinesRoseobacterSequence Analysis, DNAStaining and LabelingBenzothiazolesDiaminesDNA, BacterialDNA, Ribosomal SpacerFluorescent DyesOrganic ChemicalsQuinolinesSYBR Green I

Identifiers

PMID19801463
PMCPMC2786420

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.