ArticleApplied and environmental microbiology2009
Strain-specific genotyping of Bifidobacterium animalis subsp. lactis by using single-nucleotide polymorphisms, insertions, and deletions.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
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- Genetic engineering of gut commensals: heterologous protein expression inCurrent research in microbial sciences · 2026Article
- Development and multi-platform validation of a pan-genomic-driven PMA-qPCR method for the precise quantification of viableFrontiers in microbiology · 2026Article
- Article
- Compositional Quality and Possible Gastrointestinal Performance of Marketed Probiotic Supplements.Probiotics and antimicrobial proteins · 2022Article
- Isolation and Characterization of Commensal Bifidobacteria Strains in Gut Microbiota of Neonates Born Preterm: A Prospective Longitudinal Study.Microorganisms · 2022Article
- Editorial: Authenticity of Probiotic Foods and Dietary Supplements.Frontiers in microbiology · 2021Article
- Development of a High-Resolution Single-Nucleotide Polymorphism Strain-Typing Assay Using Whole Genome-Based Analyses for theMicroorganisms · 2020Article
- Optimization of Viability Treatment Essential for Accurate Droplet Digital PCR Enumeration of Probiotics.Frontiers in microbiology · 2020Article
- Dissecting the Evolutionary Development of the SpeciesApplied and environmental microbiology · 2019Article
- Droplet Digital PCR Is an Improved Alternative Method for High-Quality Enumeration of Viable Probiotic Strains.Frontiers in microbiology · 2019Article
- Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline.Applied and environmental microbiology · 2018Article
- Tracking the Taxonomy of the Genus Bifidobacterium Based on a Phylogenomic Approach.Applied and environmental microbiology · 2018Article
- Absolute Enumeration of Probiotic StrainsFrontiers in microbiology · 2018Article
- P4P: a peptidome-based strain-level genome comparison web tool.Nucleic acids research · 2017Article
- Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases.Frontiers in microbiology · 2016Article
- Catabolism of glucose and lactose in Bifidobacterium animalis subsp. lactis, studied by 13C Nuclear Magnetic Resonance.Applied and environmental microbiology · 2013Article
- Bifidobacterium animalis subsp. lactis ATCC 27673 is a genomically unique strain within its conserved subspecies.Applied and environmental microbiology · 2013Article
- Comparative genomics of Bifidobacterium animalis subsp. lactis reveals a strict monophyletic bifidobacterial taxon.Applied and environmental microbiology · 2013Article
- Insights into the ropy phenotype of the exopolysaccharide-producing strain Bifidobacterium animalis subsp. lactis A1dOxR.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several probiotic strains of Bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. The strain specificity of these characteristics demands techniques with high discriminatory power to differentiate among strains. However, to date, molecular approaches, such as pulsed-field gel electrophoresis and randomly amplified polymorphic DNA-PCR, have been ineffective at achieving strain separation due to the monomorphic nature of this subspecies. Previously, sequencing and comparison of two B. animalis subsp. lactis genomes (DSMZ 10140 and Bl-04) confirmed this high level of sequence similarity, identifying only 47 single-nucleotide polymorphisms (SNPs) and four insertions and/or deletions (INDELs) between them. In this study, we hypothesized that a sequence-based typing method targeting these loci would permit greater discrimination between strains than previously attempted methods. Sequencing 50 of these loci in 24 strains of B. animalis subsp. lactis revealed that a combination of nine SNPs/INDELs could be used to differentiate strains into 14 distinct genotypic groups. In addition, the presence of a nonsynonymous SNP within the gene encoding a putative glucose uptake protein was found to correlate with the ability of certain strains to transport glucose and to grow rapidly in a medium containing glucose as the sole carbon source. The method reported here can be used in clinical, regulatory, and commercial applications requiring identification of B. animalis subsp. lactis at the strain level.
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