ArticleEukaryotic cell2010
Leishmania major MPK7 protein kinase activity inhibits intracellular growth of the pathogenic amastigote stage.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Five-layer systems analysis ofeLife · 2026Article
- Chemical genetics reveals Leishmania KKT2 and CRK9 kinase activity is required for cell cycle progression.PLoS pathogens · 2026Article
- Activity-based chemoproteomic profiling reveals the active kinome ofFrontiers in pharmacology · 2025Article
- Distinguishing functions of trypanosomatid protein kinases.Trends in parasitology · 2022Review
- Promastigote-to-Amastigote Conversion inPathogens (Basel, Switzerland) · 2022Review
- Review
- Arginase/nitric oxide modifications using live non-pathogenicJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- TheFrontiers in cellular and infection microbiology · 2021Article
- Towards discovery of new leishmanicidal scaffolds able to inhibitJournal of enzyme inhibition and medicinal chemistry · 2020Article
- An overview of biochemically characterized drug targets in metabolic pathways of Leishmania parasite.Parasitology research · 2020Review
- Biochemical characterization and chemical validation of Leishmania MAP Kinase-3 as a potential drug target.Scientific reports · 2019Article
- Leishmania phosphatase PP5 is a regulator of HSP83 phosphorylation and essential for parasite pathogenicity.Parasitology research · 2018Article
- Emerging therapeutic targets for treatment of leishmaniasis.Expert opinion on therapeutic targets · 2018Review
- Identification of novel leishmanicidal molecules by virtual and biochemical screenings targeting Leishmania eukaryotic translation initiation factor 4A.PLoS neglected tropical diseases · 2018Article
- Characterization of the Protein Tyrosine Phosphatase LmPRL-1 Secreted by Leishmania major via the Exosome Pathway.Infection and immunity · 2017Article
- Carboxylate derivatives of tributyltin (IV) complexes as anticancer and antileishmanial agents.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2017Article
- Deletion of mitogen-activated protein kinase 1 inhibits development and growth of Toxoplasma gondii.Parasitology research · 2016Article
- Implication of different domains of the Leishmania major metacaspase in cell death and autophagy.Cell death & disease · 2015Article
- Fitness and Phenotypic Characterization of Miltefosine-Resistant Leishmania major.PLoS neglected tropical diseases · 2015Article
- Sterol biosynthesis is required for heat resistance but not extracellular survival in leishmania.PLoS pathogens · 2014Article
Corrections and comments
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the infectious cycle, protozoan parasites of the genus Leishmania undergo several adaptive differentiation steps that are induced by environmental factors and crucial for parasite infectivity. Genetic analyses of signaling proteins underlying Leishmania stage differentiation are often rendered difficult due to lethal null mutant phenotypes. Here we used a transgenic strategy to gain insight into the functions of the mitogen-activated Leishmania major protein kinases LmaMPK7 and LmaMPK10 in parasite virulence. We established L. major and Leishmania donovani lines expressing episomal green fluorescent protein (GFP)-LmaMPK7 and GFP-LmaMPK10 fusion proteins. The transgenic lines were normal in promastigote morphology, growth, and the ability to differentiate into metacyclic and amastigote stages. While parasites expressing GFP-LmaMPK10 showed normal infectivity by mouse footpad analysis and macrophage infection assays, GFP-LmaMPK7 transgenic parasites displayed a strong delay in lesion formation and reduced intracellular parasite growth. Significantly, the effects of GFP-LmaMPK7 on virulence and proliferation were due exclusively to protein kinase activity, as the overexpression of two kinase-dead mutants had no effect on parasite infectivity. GFP-LmaMPK7 transgenic L. donovani cells revealed a reversible, stage-specific growth defect in axenic amastigotes that was independent of cell death but linked to nonsynchronous growth arrest and a significant reduction of de novo protein biosynthesis. Our data suggest that LmaMPK7 protein kinase activity may be implicated in parasite growth control and thus relevant for the development of nonproliferating stages during the infectious cycle.
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