ArticleJournal of bacteriology2009
A family of transcriptional antitermination factors necessary for synthesis of the capsular polysaccharides of Bacteroides fragilis.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 58 citations in OpenAlex.
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- Adaptations in gut Bacteroidales facilitate stable co-existence with their lytic bacteriophages.Gut microbes · 2025Article
- Lessons from the model gut BacteroidalesJournal of bacteriology · 2025Review
- Bacteroides expand the functional versatility of a conserved transcription factor and transcribed DNA to program capsule diversity.Nature communications · 2024Article
- Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance.Nature communications · 2024Article
- Precision run-on sequencing (PRO-seq) for microbiome transcriptomics.Nature microbiology · 2024Article
- Single-cell analysis of multiple invertible promoters reveals differential inversion rates as a strong determinant of bacterial population heterogeneity.Science advances · 2023Article
- Genetic tools for the redirection of the central carbon flow towards the production of lactate in the human gut bacterium Phocaeicola (Bacteroides) vulgatus.Applied microbiology and biotechnology · 2022Article
- Bacteroidales species in the human gut are a reservoir of antibiotic resistance genes regulated by invertible promoters.NPJ biofilms and microbiomes · 2022Article
- Metamorphic proteins under a computational microscope: Lessons from a fold-switching RfaH protein.Computational and structural biotechnology journal · 2022Review
- Phase-variable bacteria simultaneously express multiple capsules.Microbiology (Reading, England) · 2021Article
- Site-Specific Recombination - How Simple DNA Inversions Produce Complex Phenotypic Heterogeneity in Bacterial Populations.Trends in genetics : TIG · 2021Review
- Phase-variable capsular polysaccharides and lipoproteins modify bacteriophage susceptibility in Bacteroides thetaiotaomicron.Nature microbiology · 2020Article
- Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.mBio · 2020Article
- Distinctive Features of PipX, a Unique Signaling Protein of Cyanobacteria.Life (Basel, Switzerland) · 2020Review
- NusG, an Ancient Yet Rapidly Evolving Transcription Factor.Frontiers in microbiology · 2020Review
- Ancient Transcription Factors in the News.mBio · 2019Review
- Promoter orientation of the immunomodulatoryGut microbes · 2019Article
- Processive Antitermination.Microbiology spectrum · 2018Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A single strain of Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, designated PSA to PSH. These polysaccharides are synthesized by-products encoded by eight separate polysaccharide biosynthesis loci. The genetic architecture of each of these eight loci is similar, including the fact that the first gene of each locus is a paralog of the first gene of each of the other PS loci. These proteins are designated the UpxY family, where x is replaced by a to h, depending upon the polysaccharide locus from which it is produced. Mutational analysis of three separate upxY genes demonstrated that they are necessary and specific for transcription of their respective polysaccharide biosynthesis operon and that they function in trans. Transcriptional reporter constructs, reverse transcriptase PCR, and deletion analysis demonstrated that the UpxYs do not affect initiation of transcription, but rather prevent premature transcriptional termination within the 5' untranslated region between the promoter and the upxY gene. The UpxYs have conserved motifs that are present in NusG and NusG-like proteins. Mutation of two conserved residues within the conserved KOW motif abrogated UpaY activity, further confirming that these proteins belong to the NusG-like (NusG(SP)) family. Alignment of highly similar UpxYs led to the identification of a small region of these proteins predicted to confer specificity for their respective loci. Construction of an upaY-upeY hybrid that produced a protein in which a 17-amino-acid segment of UpaY was changed to that of UpeY altered UpaY's specificity, as it was now able to function in transcriptional antitermination of the PSE biosynthesis operon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.