ArticleJournal of chromatography. A2009
Protein adsorption and transport in dextran-modified ion-exchange media. I: adsorption.
Article in Journal of chromatography. A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 107 citations in OpenAlex.
- Poly(lactide)-Based Materials Modified with Biomolecules: A Review.Materials (Basel, Switzerland) · 2024Review
- Article
- The use of predictive models to develop chromatography-based purification processes.Frontiers in bioengineering and biotechnology · 2022Review
- Increasing immunoglobulin G adsorption in dextran-grafted protein A gels.Engineering in life sciences · 2021Article
- Ionic strength-dependent changes in tentacular ion exchangers with variable ligand density. I. Structural properties.Journal of chromatography. A · 2016Article
- Determinants of protein elution rates from preparative ion-exchange adsorbents.Journal of chromatography. A · 2016Article
- Characterization of cross-linked cellulosic ion-exchange adsorbents: 2. Protein sorption and transport.Journal of chromatography. A · 2016Article
- Fluorescence recovery after photobleaching investigation of protein transport and exchange in chromatographic media.Journal of chromatography. A · 2014Article
- Characterization of cross-linked cellulosic ion-exchange adsorbents: 1. Structural properties.Journal of chromatography. A · 2013Article
- Protein adsorption and transport in polymer-functionalized ion-exchangers.Journal of chromatography. A · 2011Review
- Protein adsorption and transport in dextran-modified ion-exchange media. III. Effects of resin charge density and dextran content on adsorption and intraparticle uptake.Journal of chromatography. A · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Adsorption behavior is compared on a traditional agarose-based ion-exchange resin and on two dextran-modified resins, using three proteins to examine the effect of protein size. The latter resins typically exhibit higher static capacities at low ionic strengths and electron microscopy provides direct visual evidence supporting the view that the higher static capacities are due to the larger available binding volume afforded by the dextran. However, isocratic retention experiments reveal that the larger proteins can be almost completely excluded from the dextran layer at high ionic strengths, potentially leading to significant losses in static capacity at relevant column loading conditions. Knowledge of resin and protein properties is used to estimate physical limits on the static capacities of the resins in order to provide a meaningful interpretation of the observed static capacities. Results of such estimates are consistent with the expectation that available surface area is limiting for traditional resins. In dextran-modified media, however, the volume of the dextran layer appears to limit adsorption when the protein charge is low relative to the resin charge, but the protein-resin electroneutrality may be limiting when the protein charge is relatively high. Such analyses may prove useful for semiquantitative prediction of maximum static capacities and selection of operating conditions when combined with protein transport information.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.