ArticleBioconjugate chemistry2009
Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.
Article in Bioconjugate chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Ultrabright and Serum-Stable Squaraine Dyes.Journal of medicinal chemistry · 2020Article
- Small molecule fluorophore and copolymer RGD peptide conjugates for ex vivo two-photon fluorescence tumor vasculature imaging.Biomaterials · 2012Article
- Two-photon fluorescence vascular bioimaging with new bioconjugate probes selective toward the vascular endothelial growth factor receptor 2.Bioconjugate chemistry · 2011Article
- Folate receptor-targeted aggregation-enhanced near-IR emitting silica nanoprobe for one-photon in vivo and two-photon ex vivo fluorescence bioimaging.Bioconjugate chemistry · 2011Article
- Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe.Journal of fluorescence · 2011Article
- Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing.ACS applied materials & interfaces · 2010Article
- High-fidelity hydrophilic probe for two-photon fluorescence lysosomal imaging.Journal of the American Chemical Society · 2010Article
- Folate receptor targeting silica nanoparticle probe for two-photon fluorescence bioimaging.Biomedical optics express · 2010Article
- Donor-acceptor-donor fluorene derivatives for two-photon fluorescence lysosomal imaging.The Journal of organic chemistry · 2010Article
- Fluorescence Sensing of Zinc and Mercury Ions with Hydrophilic 1,2,3-Triazolyl Fluorene Probes.Chemistry of materials : a publication of the American Chemical Society · 2010Article
- Intravital microscopy as a tool to study drug delivery in preclinical studies.Advanced drug delivery reviewsReview
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Closely involved in the progression of nonlinear bioimaging is the development of optical probes for investigating biological function and activity. Introduction of new fluorescent compounds possessing enhanced nonlinearities is essential for advancing the utility of two-photon absorption (2PA) processes in the biological sciences. Herein, we report the synthesis of fluorene-based fluorophores tailored for multiphoton imaging, incorporating the succinimidyl ester and thioester functionality as reactive linkers for further coupling with a wide variety of biologically relevant molecules. The succinimidyl ester amine reactive probe was conjugated with the cyclic peptide RGDfK and polyclonal antirat IgG protein. Upon conjugation, the basic molecular architecture and photophysical properties of the active 2PA chromophore remain unchanged. Conventional and two-photon fluorescence microscopy (2PFM) imaging of COS-7 and HeLa cells, incubated with either the fluorene-RGD peptide conjugate or the fluorene-IgG conjugate, was demonstrated. The fluorene-IgG conjugate was used to image cell spindles at early mitotic developmental stages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.