ReviewMethods in molecular biology (Clifton, N.J.)2009
Gel electrophoresis assays for analyzing DNA double-strand breaks in Saccharomyces cerevisiae at various spatial resolutions.
Review in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
- Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2.Nucleic acids research · 2026Article
- Distinct and overlapping roles of MutLγ, Mus81-Mms4, and STR in meiotic Holliday junction processing.Nature communications · 2026Article
- Recruitment of Mre11 to recombination sites during meiosis.Nature communications · 2026Article
- RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis.Nature communications · 2026Article
- Dimerization of thebioRxiv : the preprint server for biology · 2026Article
- Article
- ERC accumulation depletes Sir2 from rDNA and induces cellular senescence by rDNA destabilization.Nucleic acids research · 2025Article
- Recombinase-independent chromosomal rearrangements between dispersed inverted repeats in Saccharomyces cerevisiae meiosis.Nucleic acids research · 2023Article
- Dna2 removes toxic ssDNA-RPA filaments generated from meiotic recombination-associated DNA synthesis.Nucleic acids research · 2023Article
- The S. cerevisiae m6A-reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts.Nucleic acids research · 2023Article
- The Zip4 protein directly couples meiotic crossover formation to synaptonemal complex assembly.Genes & development · 2022Article
- Article
- Structural and functional characterization of the Spo11 core complex.Nature structural & molecular biology · 2021Article
- Chromosome-autonomous feedback down-regulates meiotic DNA break competence upon synaptonemal complex formation.Genes & development · 2020Article
- Regulation of the MLH1-MLH3 endonuclease in meiosis.Nature · 2020Article
- Article
- Timing of appearance of new mutations during yeast meiosis and their association with recombination.Current genetics · 2020Article
- The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes.PLoS genetics · 2018Article
- Article
- Programming sites of meiotic crossovers using Spo11 fusion proteins.Nucleic acids research · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Meiotic recombination is triggered by programmed DNA double-strand breaks (DSBs), which are catalyzed by Spo11 protein in a type II topoisomerase-like manner. Meiotic DSBs can be detected directly using physical assays (gel electrophoresis, Southern blotting, and indirect end-labeling) applied to samples of genomic DNA from sporulating cultures of budding and fission yeast. Such assays are extremely useful for quantifying and characterizing many aspects of the initiation of meiotic recombination, including the timing of DSB formation relative to other events, the distribution of DSBs across the genome, and the influence on DSB formation of mutations in recombination factors and other gene products. By varying the type of gel electrophoresis and other parameters, the spatial resolution of DSB analysis can range from single nucleotides up to whole yeast chromosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.