ArticleProtein science : a publication of the Protein Society2009
Structural insight into the interaction of proteins containing NPF, DPF, and GPF motifs with the C-terminal EH-domain of EHD1.
Article in Protein science : a publication of the Protein Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 30 citations in OpenAlex.
- A Quarter Century of EHD Protein Research: From Endosomal Recycling to Ciliopathies.Traffic (Copenhagen, Denmark) · 2026Review
- Structures of EHD2 filaments on curved membranes provide a model for caveolar neck stabilization.Nature communications · 2026Article
- The endocytic fission protein EHD1 interacts with tubulin and regulates microtubule function.Biochimica et biophysica acta. Molecular cell research · 2026Article
- The non-catalytic DNA polymerase ε subunit is an NPF motif recognition protein.Nature communications · 2025Article
- Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network.Nature communications · 2025Article
- Coronin2A links actin-based endosomal processes to the EHD1 fission machinery.Molecular biology of the cell · 2022Article
- Differential requirements for the Eps15 homology domain proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis.Traffic (Copenhagen, Denmark) · 2022Article
- Completing the family of human Eps15 homology domains: Solution structure of the internal Eps15 homology domain of γ-synergin.Protein science : a publication of the Protein Society · 2022Article
- Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding.Nature communications · 2021Article
- Targeting SMYD3 to Sensitize Homologous Recombination-Proficient Tumors to PARP-Mediated Synthetic Lethality.iScience · 2020Article
- Sorting nexin 17 (SNX17) links endosomal sorting to Eps15 homology domain protein 1 (EHD1)-mediated fission machinery.The Journal of biological chemistry · 2020Article
- Article
- The enigmatic endosome - sorting the ins and outs of endocytic trafficking.Journal of cell science · 2018Review
- Structural insights into the activation mechanism of dynamin-like EHD ATPases.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF Peptides.The Journal of biological chemistry · 2016Article
- Molecular dynamics simulation of the interactions between EHD1 EH domain and multiple peptides.Journal of Zhejiang University. Science. B · 2015Article
- Role of the EHD2 unstructured loop in dimerization, protein binding and subcellular localization.PloS one · 2015Article
- Structured cyclic peptides that bind the EH domain of EHD1.Biochemistry · 2014Article
- Biochemical and functional characterization of CpMuc4, a Cryptosporidium surface antigen that binds to host epithelial cells.Molecular and biochemical parasitology · 2014Article
- A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode.PloS one · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Eps15 homology (EH)-domain containing proteins are regulators of endocytic membrane trafficking. EH-domain binding to proteins containing the tripeptide NPF has been well characterized, but recent studies have shown that EH-domains are also able to interact with ligands containing DPF or GPF motifs. We demonstrate that the three motifs interact in a similar way with the EH-domain of EHD1, with the NPF motif having the highest affinity due to the presence of an intermolecular hydrogen bond. The weaker affinity for the DPF and GPF motifs suggests that if complex formation occurs in vivo, they may require high ligand concentrations, the presence of successive motifs and/or specific flanking residues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.