ArticlePLoS genetics2009
Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during Caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis.
Article in PLoS genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 71 citations in OpenAlex.
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- ThebioRxiv : the preprint server for biology · 2025Article
- The actin assembly regulatorbioRxiv : the preprint server for biology · 2025Article
- High-density SNP-based linkage map construction and QTL analysis for growth-related traits inFrontiers in genetics · 2025Article
- Adaptor protein Abelson interactor 1 in homeostasis and disease.Cell communication and signaling : CCS · 2024Review
- Article
- Filopodial protrusion driven by density-dependent Ena-TOCA-1 interactions.Journal of cell science · 2024Article
- Actin and CDC-42 contribute to nuclear migration through constricted spaces in C. elegans.Development (Cambridge, England) · 2023Article
- FLN-2 functions in parallel to LINC complexes and Cdc42/actin pathways during P-cell nuclear migration through constricted spaces inbioRxiv : the preprint server for biology · 2023Article
- WAVE facilitates polarized E-cadherin transport.Molecular biology of the cell · 2023Article
- Cdc42 functions as a regulatory node for tumour-derived microvesicle biogenesis.Journal of extracellular vesicles · 2021Article
- New insights into the molecular mechanisms of axon guidance receptor regulation and signaling.Current topics in developmental biology · 2021Review
- RhoGAP RGA-8 supports morphogenesis inBiology open · 2020Article
- A network of nuclear envelope proteins and cytoskeletal force generators mediates movements of and within nuclei throughoutExperimental biology and medicine (Maywood, N.J.) · 2019Review
- WAVE regulates Cadherin junction assembly and turnover during epithelial polarization.Developmental biology · 2018Article
- Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning.Developmental cell · 2017Article
- Cdc42 in actin dynamics: An ordered pathway governed by complex equilibria and directional effector handover.Small GTPases · 2017Review
- Adherens Junctions on the Move-Membrane Trafficking of E-Cadherin.Cold Spring Harbor perspectives in biology · 2017Review
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Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
Abstract
The TOCA family of F-BAR-containing proteins bind to and remodel lipid bilayers via their conserved F-BAR domains, and regulate actin dynamics via their N-Wasp binding SH3 domains. Thus, these proteins are predicted to play a pivotal role in coordinating membrane traffic with actin dynamics during cell migration and tissue morphogenesis. By combining genetic analysis in Caenorhabditis elegans with cellular biochemical experiments in mammalian cells, we showed that: i) loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. Oocyte endocytosis defects correlated well with reduced egg production in these mutants. ii) CeTOCA proteins localize to cell-cell junctions and are required for proper embryonic morphogenesis, to position hypodermal cells and to organize junctional actin and the junction-associated protein AJM-1. iii) Double mutant analysis indicated that the toca genes act in the same pathway as the nematode homologue of N-WASP/WASP, wsp-1. Furthermore, mammalian TOCA-1 and C. elegans CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. Thus, we propose that TOCA proteins control tissues morphogenesis by coordinating Clathrin-dependent membrane trafficking with WAVE and N-WASP-dependent actin-dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.