ArticlePLoS genetics2009
Compensatory evolution of gene regulation in response to stress by Escherichia coli lacking RpoS.
Article in PLoS genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 99 citations in OpenAlex.
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- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
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- Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli.AMB Express · 2020Article
- Evolutionary Repair Experiments as a Window to the Molecular Diversity of Life.Current biology : CB · 2020Review
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Rapid Accumulation of Motility-Activating Mutations in Resting Liquid Culture of Escherichia coli.Journal of bacteriology · 2019Article
- Rapid decline of bacterial drug-resistance in an antibiotic-free environment through phenotypic reversion.eLife · 2019Article
- Novel approach to quantitative spatial gene expression uncovers genetic stochasticity in the developing Drosophila eye.Evolution & development · 2019Article
- Within-Host Adaptation Mediated by Intergenic Evolution in Pseudomonas aeruginosa.Genome biology and evolution · 2019Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- Random sequences rapidly evolve into de novo promoters.Nature communications · 2018Article
- Article
- Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements.Microbial biotechnology · 2017Article
- Subfunctionalization influences the expansion of bacterial multidrug antibiotic resistance.BMC genomics · 2017Article
- The adaptive landscape of wildtype and glycosylation-deficient populations of the industrial yeast Pichia pastoris.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The RpoS sigma factor protein of Escherichia coli RNA polymerase is the master transcriptional regulator of physiological responses to a variety of stresses. This stress response comes at the expense of scavenging for scarce resources, causing a trade-off between stress tolerance and nutrient acquisition. This trade-off favors non-functional rpoS alleles in nutrient-poor environments. We used experimental evolution to explore how natural selection modifies the regulatory network of strains lacking RpoS when they evolve in an osmotically stressful environment. We found that strains lacking RpoS adapt less variably, in terms of both fitness increase and changes in patterns of transcription, than strains with functional RpoS. This phenotypic uniformity was caused by the same adaptive mutation in every independent population: the insertion of IS10 into the promoter of the otsBA operon. OtsA and OtsB are required to synthesize the osmoprotectant trehalose, and transcription of otsBA requires RpoS in the wild-type genetic background. The evolved IS10 insertion rewires expression of otsBA from RpoS-dependent to RpoS-independent, allowing for partial restoration of wild-type response to osmotic stress. Our results show that the regulatory networks of bacteria can evolve new structures in ways that are both rapid and repeatable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.