ArticleLeukemia2010
Stem cell traits in long-term co-culture revealed by time-lapse imaging.
Article in Leukemia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Human Muscle-Derived Vascular Stem Cells Can Support Hematopoietic Stem/Progenitor Cells In Vitro.Stem cells international · 2025Article
- Mild hypoxia and human bone marrow mesenchymal stem cells synergistically enhance expansion and homing capacity of human cord blood CD34+ stem cells.Iranian journal of basic medical sciences · 2018Article
- Automated tracking and quantification of angiogenic vessel formation in 3D microfluidic devices.PloS one · 2017Article
- Apoptosis, DAP-Kinase1 Expression and the Influences of Cytokine Milieu and Mesenchymal Stromal Cells on Ex Vivo Expansion of Umbilical Cord Blood-Derived Hematopoietic Stem Cells.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2016Article
- Label-free imaging to study phenotypic behavioural traits of cells in complex co-cultures.Scientific reports · 2016Article
- Altered mesenchymal niche cells impede generation of normal hematopoietic progenitor cells in leukemic bone marrow.Leukemia · 2016Article
- Small-molecule inhibitors targeting INK4 protein p18(INK4C) enhance ex vivo expansion of haematopoietic stem cells.Nature communications · 2015Article
- Mesenchymal stem cells as a feeder layer can prevent apoptosis of expanded hematopoietic stem cells derived from cord blood.International journal of molecular and cellular medicine · 2014Article
- Bid is a positive regulator for donor-derived lymphoid cell regeneration in γ-irradiated recipients.Experimental hematology · 2011Article
- Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
A deeper understanding of stem cell niche engagement and subsequent behaviors would be enhanced by technologies enabling the tracking of individual stem cells at the clonal level in long-term co-culture (LTC), which mimics the complexity of the bone marrow microenvironment in vivo. Here, we report the application of time-lapse imaging with intermittent fluorescence for tracking well-defined populations of GFP(+) murine hematopoietic stem cells (HSCs) using LTC for >5 weeks. Long-term (LT) and short-term (ST) repopulating HSCs and hematopoietic progenitor cells (HPCs) were compared. The transition from cobblestone areas (CAs) under the stromal cell mantle into dispersed migrating cells on top of the stroma (COS) were directly observed. The ST-HSC and LT-HSC were able to initiate multiple waves of CA formation and COS expansion beyond 2 and 4 weeks, respectively. Retrospective tracking of individual CA forming cell (CAFC) revealed a preference for residing under stroma before the first division and a longer interval before first division for LT-HSC. Inability to maintain quiescence in subsequent divisions was revealed. Our study represents an important starting point from which the LTC system can be augmented to provide a better in vitro model for bone marrow stem cell niches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.