ArticleNature protocols2009
Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
98 citing papers in PubMed.
- Article
- Machine-guided dual-objective protein engineering for deimmunization and therapeutic functions.Cell systems · 2025Article
- C7-Substituted Quinolines as Potent Inhibitors of AdeG Efflux Pumps inACS infectious diseases · 2025Article
- Unconstrained Precision Mitochondrial Genome Editing with αDdCBEs.Human gene therapy · 2024Article
- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- Neuroepigenetic Editing.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Corneal gene therapy: Structural and mechanistic understanding.The ocular surface · 2023Article
- Targeted DNA Demethylation: Vectors, Effectors and Perspectives.Biomedicines · 2023Review
- Stem Cell-Based Therapeutic Approaches in Genetic Diseases.Advances in experimental medicine and biology · 2023Article
- Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Multidimensional control of therapeutic human cell function with synthetic gene circuits.Science (New York, N.Y.) · 2022Article
- Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivo.Nature communications · 2022Article
- Genome-wide functional perturbation of human microsatellite repeats using engineered zinc finger transcription factors.Cell genomics · 2022Article
- Puma, noxa, p53, and p63 differentially mediate stress pathway induced apoptosis.Cell death & disease · 2021Article
- Current status of the application of gene editing in pigs.The Journal of reproduction and development · 2021Review
- INDEL detection, the 'Achilles heel' of precise genome editing: a survey of methods for accurate profiling of gene editing induced indels.Nucleic acids research · 2020Review
- Advance genome editing technologies in the treatment of human diseases: CRISPR therapy (Review).International journal of molecular medicine · 2020Review
- Programmable T7-based synthetic transcription factors.Nucleic acids research · 2018Article
- Neuroepigenetic Editing.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design.Cancer cell international · 2018Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly applicable method for targeted, efficient modification of eukaryotic genomes. In recent work, we described OPEN (oligomerized pool engineering), an 'open-source,' combinatorial selection-based method for engineering zinc-finger arrays that function well as ZFNs. We have also shown in direct comparisons that the OPEN method has a higher success rate than previously described 'modular-assembly' methods for engineering ZFNs. OPEN selections are carried out in Escherichia coli using a bacterial two-hybrid system and do not require specialized equipment. Here we provide a detailed protocol for carrying out OPEN to engineer zinc-finger arrays that have a high probability of functioning as ZFNs. Using OPEN, researchers can generate multiple, customized ZFNs in approximately 8 weeks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.