ArticleGenome research2009
Rapid identification of homologous recombinants and determination of gene copy number with reference/query pyrosequencing (RQPS).
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Perivascular cells support folliculogenesis in the developing ovary.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A perivascular niche for multipotent progenitors in the fetal testis.Nature communications · 2018Article
- Quantitative Measurement of PARD3 Copy Number Variations in Human Neural Tube Defects.Cellular and molecular neurobiology · 2018Article
- Second-generation Notch1 activity-trap mouse line (N1IP::CreHI) provides a more comprehensive map of cells experiencing Notch1 activity.Development (Cambridge, England) · 2015Article
- Overview of genetic tools and techniques to study Notch signaling in mice.Methods in molecular biology (Clifton, N.J.) · 2014Review
- The extracellular domain of Notch2 increases its cell-surface abundance and ligand responsiveness during kidney development.Developmental cell · 2013Article
- In vivo visualization of Notch1 proteolysis reveals the heterogeneity of Notch1 signaling activity in the mouse cochlea.PloS one · 2013Article
- Dll1- and dll4-mediated notch signaling are required for homeostasis of intestinal stem cells.Gastroenterology · 2011Article
- Notch1 loss of heterozygosity causes vascular tumors and lethal hemorrhage in mice.The Journal of clinical investigation · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Manipulating the mouse genome is a widespread technology with important applications in many biological fields ranging from cancer research to developmental biology. Likewise, correlations between copy number variations (CNVs) and human diseases are emerging. We have developed the reference-query pyrosequencing (RQPS) method, which is based on quantitative pyrosequencing and uniquely designed probes containing single nucleotide variations (SNVs), to offer a simple and affordable genotyping solution capable of identifying homologous recombinants independent of the homology arm size, determining the micro-amplification status of endogenous human loci, and quantifying virus/transgene copy number in experimental or commercial species. In addition, we also present a simple pyrosequencing-based protocol that could be used for the enrichment of homologous recombinant embryonic stem (ES) cells.
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Registered trials
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