ArticleInvestigative ophthalmology & visual science2010
Elevated albumin in retinas of monkeys with experimental glaucoma.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Suppression of inner blood-retinal barrier breakdown and pathogenic Müller glia activation in ischemia retinopathy by myeloid cell depletion.Journal of neuroinflammation · 2024Article
- Transthyretin proteoforms of intraocular origin in human subretinal fluid.Experimental eye research · 2022Article
- Alterations in proteome of human sclera associated with primary open-angle glaucoma involve proteins participating in regulation of the extracellular matrix.Molecular vision · 2020Article
- Quantitative profiling of regional protein expression in rat retina after partial optic nerve transection using fluorescence difference two‑dimensional gel electrophoresis.Molecular medicine reports · 2019Article
- NrCAM is a marker for substrate-selective activation of ADAM10 in Alzheimer's disease.EMBO molecular medicine · 2019Article
- Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits.Journal of ophthalmology · 2015Article
- A decade of proteomics studies of glaucomatous neurodegeneration.Proteomics. Clinical applications · 2014Review
- A proteomics view of the molecular mechanisms and biomarkers of glaucomatous neurodegeneration.Progress in retinal and eye research · 2013Review
- Anterior segment alterations and comparative aqueous humor proteomics in the buphthalmic rabbit (an American Ophthalmological Society thesis).Transactions of the American Ophthalmological Society · 2011Review
- Protein changes in the retina following experimental retinal detachment in rabbits.Molecular vision · 2011Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeTo establish the identity of a prominent protein, approximately 70 kDa, that is markedly increased in the retina of monkeys with experimental glaucoma compared with the fellow control retina, the relationship to glaucoma severity, and its localization in the retina.
methodsRetinal extracts were subjected to 2-D gel electrophoresis to identify differentially expressed proteins. Purified peptides from the abundant 70 kDa protein were analyzed and identified by liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) separation, and collision-induced dissociation sequencing. Protein identity was performed on MASCOT (Matrix Science, Boston, MA) and confirmed by Western blot. The relationship between the increase in this protein and glaucoma severity was investigated by regression analyses. Protein localization in retina was evaluated by immunohistochemistry with confocal imaging.
resultsThe abundant protein was identified as Macaca mulatta serum albumin precursor (67 kDa) from eight non-overlapping proteolytic fragments, and the identity was confirmed by Western blot. The average increase in retinal albumin content was 2.3 fold (P = 0.015). In glaucoma eyes, albumin was localized to some neurons of the inner nuclear layer, in the inner plexiform layer, and along the vitreal surface, but it was only found in blood vessels in control retinas.
conclusionsAlbumin is the abundant protein found in the glaucomatous monkey retinas. The increased albumin is primarily localized to the inner retina where oxidative damage associated with experimental glaucoma is known to be prominent. Since albumin is a major antioxidant, the increase of albumin in the retinas of eyes with experimental glaucoma may serve to protect the retina against oxidative damage.
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