ArticleDNA repair2009
A negatively charged residue in place of histone H3K56 supports chromatin assembly factor association but not genotoxic stress resistance.
Article in DNA repair, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- A Rfa1-MN-based system reveals new factors involved in the rescue of broken replication forks.PLoS genetics · 2025Article
- Characterization of HMGA2 variants expands the spectrum of Silver-Russell syndrome.JCI insight · 2024Article
- The Role of Histone Modification in DNA Replication-Coupled Nucleosome Assembly and Cancer.International journal of molecular sciences · 2023Review
- Review
- Cell cycle-dependent changes in H3K56ac in human cells.Cell cycle (Georgetown, Tex.) · 2015Article
- A reversible histone H3 acetylation cooperates with mismatch repair and replicative polymerases in maintaining genome stability.PLoS genetics · 2013Article
- A small molecule inhibitor of fungal histone acetyltransferase Rtt109.Bioorganic & medicinal chemistry letters · 2013Article
- Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56.The Journal of biological chemistry · 2012Article
- Chromatin-mediated Candida albicans virulence.Biochimica et biophysica acta · 2012Article
- Nucleosome assembly and genome integrity: The fork is the link.Bioarchitecture · 2012Article
- Histone H3K56 acetylation, CAF1, and Rtt106 coordinate nucleosome assembly and stability of advancing replication forks.PLoS genetics · 2011Article
- Catalytic activation of histone acetyltransferase Rtt109 by a histone chaperone.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Histone H3 serine 57 and lysine 56 interplay in transcription elongation and recovery from S-phase stress.PloS one · 2010Article
- Histone acetyltransferase Rtt109 is required for Candida albicans pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In fungal species, lysine 56 of newly synthesized histone H3 molecules is modified by the acetyltransferase Rtt109, which promotes resistance to genotoxic agents. To further explore how H3 K56ac contributes to genome stability, we conducted screens for suppressors of the DNA damage sensitivity of budding yeast rtt109 Delta mutants. We recovered a single extragenic suppressor mutation that efficiently restored damage resistance. The suppressor is a point mutation in the histone H3 gene HHT2, and converts lysine 56 to glutamic acid. In some ways, K56E mimics K56ac, because it suppresses other mutations that interfere with the production of H3 K56ac and restores histone binding to chromatin assembly proteins CAF-1 and Rtt106. Therefore, we demonstrate that enhanced association with chromatin assembly factors can be accomplished not only by acetylation-mediated charge neutralization of H3K56 but also by the replacement of the positively charged lysine with an acidic residue. These data suggest that removal of the positive charge on lysine 56 is the functionally important consequence of H3K56 acetylation. Additionally, the suppressive function of K56E requires the presence of a second H3 allele, because K56E impairs growth when it is the sole source of histones, even more so than does constitutive H3K56 acetylation. Our studies therefore emphasize how H3 K56ac not only promotes chromatin assembly but also leads to chromosomal malfunction if not removed following histone deposition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.