ArticleMolecular imaging
Real-time monitoring of nuclear factor kappaB activity in cultured cells and in animal models.
Article in Molecular imaging. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 71 citations in OpenAlex.
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- Frequency-specific photobiomodulation at theta and gamma enhances cognitive networks and mitigates age-related decline.GeroScience · 2026Article
- Pesticide exposure and the microbiota-gut-brain axis.The ISME journal · 2023Review
- Pixelated Microfluidics for Drug Screening on Tumour Spheroids and Ex Vivo Microdissected Tumour Explants.Cancers · 2023Article
- Neuronal Activity Reporters as Drug Screening Platforms.Micromachines · 2022Review
- Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.Nature medicine · 2022Article
- Creation of a synthesis-friendly inflammation-inducible promoter suitable for cell therapy.Integrative biology : quantitative biosciences from nano to macro · 2021Article
- Anti‑inflammatory role of microRNA‑429 in human gingival epithelial cells‑inhibition of IL‑8 production through direct binding to IKKβ mRNA.Molecular medicine reports · 2021Article
- Review
- Stable Cells with NF-κB-ZsGreen Fused Genes Created by TALEN Editing and Homology Directed Repair for Screening Anti-inflammation Drugs.Journal of inflammation research · 2021Article
- Generation of an NFκB-Driven Alpharetroviral "All-in-One" Vector Construct as a Potent Tool for CAR NK Cell Therapy.Frontiers in immunology · 2021Article
- Extracellular Vesicles Induce Mesenchymal Transition and Therapeutic Resistance in Glioblastomas through NF-κB/STAT3 Signaling.Advanced biosystems · 2020Article
- A novel positive feedback-loop between the HTLV-1 oncoprotein Tax and NF-κB activity in T-cells.Retrovirology · 2020Article
- Tracking leukemic T-cell transcriptional dynamics in vivo with a blood-based reporter assay.FEBS open bio · 2020Article
- Quercetin improves immune function in Arbor Acre broilers through activation of NF-κB signaling pathway.Poultry science · 2020Article
- Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing.Lasers in medical science · 2019Article
- A Synthetic Gene Circuit for Self-Regulating Delivery of Biologic Drugs in Engineered Tissues.Tissue engineering. Part A · 2019Article
- Design and analysis of stably integrated reporters for inducible transgene expression in human T cells and CAR NK-cell lines.BMC medical genomics · 2019Article
- Sustained NF-κB-STAT3 signaling promotes resistance to Smac mimetics in Glioma stem-like cells but creates a vulnerability to EZH2 inhibition.Cell death discovery · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Nuclear factor kappaB (NF-kappaB) is a transcription factor that plays a major role in many human disorders, including immune diseases and cancer. We designed a reporter system based on NF-kappaB responsive promoter elements driving expression of the secreted Gaussia princeps luciferase (Gluc). We show that this bioluminescent reporter is a highly sensitive tool for noninvasive monitoring of the kinetics of NF-kappaB activation and inhibition over time, both in conditioned medium of cultured cells and in the blood and urine of animals. NF-kappaB activation was successfully monitored in real time in endothelial cells in response to tumor angiogenic signaling, as well as in monocytes in response to inflammation. Further, we demonstrated dual blood monitoring of both NF-kappaB activation during tumor development as correlated to tumor formation using the NF-kappaB Gluc reporter, as well as the secreted alkaline phosphatase reporter. This NF-kappaB reporter system provides a powerful tool for monitoring NF-kappaB activity in real time in vitro and in vivo.
Indexed as
Identifiers
19796605PMC2856067W2172224990What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.