ArticleThe Journal of infectious diseases2009
Restriction fragment length polymorphism typing demonstrates substantial diversity among Pneumocystis jirovecii isolates.
Article in The Journal of infectious diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Unanticipated Global Emergence of MultiplemedRxiv : the preprint server for health sciences · 2025Article
- Estimated Burden of Fungal Infections in Oman.Journal of fungi (Basel, Switzerland) · 2020Article
- Article
- A multi-centered study ofCurrent medical mycology · 2019Article
- Article
- A Molecular Window into the Biology and Epidemiology of Pneumocystis spp.Clinical microbiology reviews · 2018Review
- Added Value of Next-Generation Sequencing for Multilocus Sequence Typing Analysis of a Pneumocystis jirovecii Pneumonia Outbreak1.Emerging infectious diseases · 2017Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Infections in the immunosuppressed host.Annals of the American Thoracic Society · 2014Review
- Pneumocystis jirovecii Rtt109, a novel drug target for Pneumocystis pneumonia in immunosuppressed humans.Antimicrobial agents and chemotherapy · 2014Article
- Outbreak of pneumocystis pneumonia in renal and liver transplant patients caused by genotypically distinct strains of Pneumocystis jirovecii.Transplantation · 2013Article
- Trimethoprim resistance of dihydrofolate reductase variants from clinical isolates of Pneumocystis jirovecii.Antimicrobial agents and chemotherapy · 2013Article
- Pneumocystis jirovecii infection: an emerging threat to patients with rheumatoid arthritis.Rheumatology (Oxford, England) · 2012Review
- Microbial antigenic variation mediated by homologous DNA recombination.FEMS microbiology reviews · 2012Review
- Outbreaks of Pneumocystis pneumonia in 2 renal transplant centers linked to a single strain of Pneumocystis: implications for transmission and virulence.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- HIV-associated Pneumocystis pneumonia.Proceedings of the American Thoracic Society · 2011Review
- Characterization of the meiosis-specific recombinase Dmc1 of pneumocystis.The Journal of infectious diseases · 2010Article
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Authors and funding
12 authors.
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Abstract
Better understanding of the epidemiology and transmission patterns of human Pneumocystis should lead to improved strategies for preventing Pneumocystis pneumonia (PCP). We have developed a typing method for Pneumocystis jirovecii that is based on restriction fragment length polymorphism (RFLP) analysis after polymerase chain reaction amplification of an approximately 1300 base-pair region of the msg gene family, which comprises an estimated 50-100 genes/genome. The RFLP pattern was reproducible in samples containing >1000 msg copies/reaction and was stable over time, based on analysis of serial samples from the same patient. In our initial analysis of 48 samples, we found that samples obtained from different individuals showed distinct banding patterns; only samples obtained from the same patient showed an identical RFLP pattern. Despite this substantial diversity, samples tended to cluster on the basis of country of origin. In an evaluation of samples obtained from an outbreak of PCP in kidney transplant recipients in Germany, RFLP analysis demonstrated identical patterns in samples that were from 12 patients previously linked to this outbreak, as well as from 2 additional patients. Our results highlight the presence of a remarkable diversity in human Pneumocystis strains. RFLP may be very useful for studying clusters of PCP in immunosuppressed patients, to determine whether there is a common source of infection.
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