ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2009
Comparative infection efficiency of Porcine reproductive and respiratory syndrome virus field isolates on MA104 cells and porcine alveolar macrophages.
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Identification of a conserved neutralizing epitope on the ASFV H240R protein by a nanobody that inhibits viral attachment.The Journal of biological chemistry · 2026Article
- Optimization and application of an immuoperoxidase monolayer assay for the detection of PRRSV.BMC veterinary research · 2025Article
- Research Progress on the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus.Animals : an open access journal from MDPI · 2025Review
- Genomic Characterization and Pathogenicity of BJEU06-1-Like PRRSV-1 ZD-1 Isolated in China.Transboundary and emerging diseases · 2023Article
- Article
- Influence of PRRSV-1 vaccination and infection on mononuclear immune cells at the maternal-fetal interface.Frontiers in immunology · 2022Article
- Article
- Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples.Journal of clinical microbiology · 2021Article
- Porcine Reproductive and Respiratory Syndrome Virus Enhances Self-Replication via AP-1-Dependent Induction of SOCS1.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15Veterinary research · 2018Article
- Structural insights into type I and type II of nsp4 porcine reproductive and respiratory syndrome virus (nsp4 PRRSV) by molecular dynamics simulations.Journal of molecular graphics & modelling · 2017Article
- Emergence of a virulent porcine reproductive and respiratory syndrome virus (PRRSV) 1 strain in Lower Austria.Porcine health management · 2016Article
- Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection.PloS one · 2016Article
- N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.Virology · 2015Article
- Porcine reproductive and respiratory syndrome virus induces IL-1β production depending on TLR4/MyD88 pathway and NLRP3 inflammasome in primary porcine alveolar macrophages.Mediators of inflammation · 2014Article
- Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163.BMC biotechnology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of Porcine reproductive and respiratory syndrome virus (PRRSV) on MA104 or MARC-145 cells is frequently used in PRRS diagnosis. However, the ability of recent field isolates to grow on these established simian cell lines has not been determined. The aim of this study was to characterize the growth of PRRSV field isolates on primary porcine alveolar macrophages (PAMs) and MA104 cells in comparison with the growth of the laboratory-adapted strain VR-2332. A cytopathic effect was observed in 70% of serum samples after 1 passage on PAMs and was verified by immunofluorescent staining or reverse transcriptase-polymerase chain reaction. Field isolate growth was observed on MA104 cells for only 1 of 50 serum samples after 14 d. Strain VR-2332 grew readily in MA104 cells [maximum titer, 10(7) TCID(50) (median tissue culture infective dose) per milliliter at 30 h] but not in PAMs (10(2) TCID(50)/mL at 72 h). These results show that PAMs are superior to simian cells for diagnostic isolation of current field PRRSV strains.
Indexed as
Identifiers
19794892PMC2705074What OpenQuestion holds
Registered trials
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