ArticleAmerican journal of physiology. Cell physiology2009
Expression of plasmid DNA in the salivary gland epithelium: novel approaches to study dynamic cellular processes in live animals.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 44 citations in OpenAlex.
- Coordination of force-generating actin-based modules stabilizes and remodels membranes in vivo.The Journal of cell biology · 2024Article
- Transduction of Salivary Gland Acinar Cells with a Novel AAV Vector 44.9.Molecular therapy. Methods & clinical development · 2020Article
- Application of advances in endocytosis and membrane trafficking to drug delivery.Advanced drug delivery reviews · 2020Review
- Cdc42 controls secretory granules morphology in rodent salivary glands in vivo.Communicative & integrative biology · 2020Article
- Intravital microscopy in mammalian multicellular organisms.Current opinion in cell biology · 2019Review
- Dynamic polyhedral actomyosin lattices remodel micron-scale curved membranes during exocytosis in live mice.Nature cell biology · 2019Article
- Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals.Molecular biology of the cell · 2019Article
- Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy.Journal of visualized experiments : JoVE · 2018Article
- Concerted actions of distinct nonmuscle myosin II isoforms drive intracellular membrane remodeling in live animals.The Journal of cell biology · 2017Article
- Imaging membrane remodeling during regulated exocytosis in live mice.Experimental cell research · 2015Review
- Imaging the dynamics of endocytosis in live mammalian tissues.Cold Spring Harbor perspectives in biology · 2014Review
- Polyethylenimine-mediated expression of transgenes in the acinar cells of rats salivary glands in vivo.Frontiers in cell and developmental biology · 2014Article
- Intravital microscopy for imaging subcellular structures in live mice expressing fluorescent proteins.Journal of visualized experiments : JoVE · 2013Article
- Imaging cell biology in live animals: ready for prime time.The Journal of cell biology · 2013Review
- From seeing to believing: labelling strategies for in vivo cell-tracking experiments.Interface focus · 2013Article
- Nanoparticle-mediated gene silencing confers radioprotection to salivary glands in vivo.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Intravital microscopy to image membrane trafficking in live rats.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Article
- Plasmid DNA is internalized from the apical plasma membrane of the salivary gland epithelium in live animals.Histochemistry and cell biology · 2012Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
The ability to dynamically image cellular and subcellular structures in a live animal and to target genes to a specific cell population in a living tissue provides a unique tool to address many biological questions in the proper physiological context. Here, we describe a powerful approach that is based on the use of rat submandibular salivary glands, which offer the possibility to easily perform intravital imaging and deliver molecules from the oral cavity, and plasmid DNA, which offers the advantage of rapid manipulations. We show that, under different experimental conditions, a reporter molecule can be rapidly expressed in specific compartments of the glands: 1) in the intercalated ducts, when plasmid DNA is administered alone, and 2) in granular ducts, striated ducts, and, to a lesser extent, acini, when plasmid DNA is mixed with replication-deficient adenovirus subtype 5 particles. Remarkably, we also found that gene expression can be directed to acinar cells when plasmid DNA is administered during isoproterenol-stimulated exocytosis, suggesting a novel mechanism of plasmid internalization regulated by compensatory endocytosis. Finally, as a practical application of these strategies, we show how the expression of fluorescently tagged molecules enables the study of the dynamics of various organelles in live animals at a resolution comparable to that achieved in cell cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.