ArticleAmerican journal of physiology. Renal physiology2009
PTH transiently increases the percent mobile fraction of Npt2a in OK cells as determined by FRAP.
Article in American journal of physiology. Renal physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Advances in fluorescence microscopy techniques to study kidney function.Nature reviews. Nephrology · 2021Review
- Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport.The Journal of biological chemistry · 2019Article
- NaPi-IIa interacting partners and their (un)known functional roles.Pflugers Archiv : European journal of physiology · 2019Review
- Loss of NHERF-1 expression prevents dopamine-mediated Na-K-ATPase regulation in renal proximal tubule cells from rat models of hypertension: aged F344 rats and spontaneously hypertensive rats.American journal of physiology. Cell physiology · 2017Article
- Effects of phospho- and calciotropic hormones on electrolyte transport in the proximal tubule.F1000Research · 2017Review
- Lipid-Bilayer Dynamics Probed by a Carbon Dot-Phospholipid Conjugate.Biophysical journal · 2016Article
- Role of Na+/H+ exchanger regulatory factor 1 in forward trafficking of the type IIa Na+-Pi cotransporter.American journal of physiology. Renal physiology · 2015Article
- NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells.The Journal of biological chemistry · 2013Article
- Activation of a non-cAMP/PKA signaling pathway downstream of the PTH/PTHrP receptor is essential for a sustained hypophosphatemic response to PTH infusion in male mice.Endocrinology · 2013Article
- Shank2 contributes to the apical retention and intracellular redistribution of NaPiIIa in OK cells.American journal of physiology. Cell physiology · 2013Article
- NHERF-1 and the regulation of renal phosphate reabsoption: a tale of three hormones.American journal of physiology. Renal physiology · 2012Article
- PTH-mediated inhibition of the renal transport of phosphate.Experimental cell research · 2012Review
- Regulation of G protein-coupled receptor function by Na+/H+ exchange regulatory factors.Pharmacological reviews · 2011Review
- Differential modulation of the molecular dynamics of the type IIa and IIc sodium phosphate cotransporters by parathyroid hormone.American journal of physiology. Cell physiology · 2011Article
- Dynamics of PTH-induced disassembly of Npt2a/NHERF-1 complexes in living OK cells.American journal of physiology. Renal physiology · 2011Article
- Cooperativity between the phosphorylation of Thr95 and Ser77 of NHERF-1 in the hormonal regulation of renal phosphate transport.The Journal of biological chemistry · 2010Article
- Role of NHERF and scaffolding proteins in proximal tubule transport.Urological research · 2010Review
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Renal sodium-dependent phosphate transporter 2a (Npt2a) binds to a number of PDZ adaptor proteins including sodium-hydrogen exchanger regulatory factor-1 (NHERF-1), which regulates its retention in the apical membrane of renal proximal tubule cells and the response to parathyroid hormone (PTH). The present experiments were designed to study the lateral mobility of enhanced green fluorescent protein (EGFP)-Npt2a in proximal tubule-like opossum kidney (OK) cells using fluorescence recovery after photobleaching (FRAP) and to determine the role of PDZ binding proteins in mediating the effects of PTH. The mobile fraction of wild-type Npt2a (EGFP-Npt2a-TRL) under basal conditions was approximately 17%. Treatment of the cells with Bis(sulfosuccinimidyl) suberate, a water-soluble cross-linker, abolished recovery nearly completely, indicating that recovery represented lateral diffusion in the plasma membrane and not the exocytosis or synthesis of unbleached transporter. Substitution of the C-terminal amino acid PDZ binding sequence TRL with AAA (EGFP-Npt2a-AAA) resulted in a nearly twofold increase in percent mobile fraction of Npt2a. Treatment of cells with PTH resulted in a rapid increase in the percent mobile fraction to >30% followed by a time-dependent decrease to baseline or below. PTH had no effect on the mobility of EGFP-Npt2a-AAA expressed in native OK cells or on wild-type EGFP-Npt2a-TRL expressed in OK-H cells deficient in NHERF-1. These findings indicate that the association of Npt2a with PDZ binding proteins limits the lateral mobility of the transporter in the apical membrane of renal proximal tubule cells. Treatment with PTH, presumably by dissociating NHERF-1/Npt2a complexes, transiently increases the mobility of Npt2a, suggesting that freeing of Npt2a from the cytoskeleton precedes PTH-mediated endocytosis.
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