ArticleJournal of clinical microbiology2009
Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- The vaginal microbiota: what have we learned after a decade of molecular characterization?PloS one · 2014Pooled it
- Altered vaginal microbiota are associated with perinatal mother-to-child transmission of HIV in African women from Burkina Faso.Journal of acquired immune deficiency syndromes (1999) · 2012Trial
- Maternal vaginal microbiome composition does not affect development of the infant gut microbiome in early life.Frontiers in cellular and infection microbiology · 2023Article
- Bacterial vaginosis: a review of approaches to treatment and prevention.Frontiers in reproductive health · 2023Review
- Composition and Stability of the Vaginal Microbiota of Pregnant Women With Inflammatory Bowel Disease.Inflammatory bowel diseases · 2022Article
- Early Neonatal Meconium Does Not Have a Demonstrable Microbiota Determined through Use of Robust Negative Controls withMicrobiology spectrum · 2021Article
- CaptureSeq: Hybridization-Based Enrichment ofMicroorganisms · 2021Article
- Article
- A common vaginal microbiota composition among breeds of Bos taurus indicus (Gyr and Nellore).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Update on cpnDB: a reference database of chaperonin sequences.Database : the journal of biological databases and curation · 2019Article
- A multiplex real-time PCR assay for routine diagnosis of bacterial vaginosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Article
- Anaerobes and bacterial vaginosis in pregnancy: virulence factors contributing to vaginal colonisation.International journal of environmental research and public health · 2014Review
- Simultaneous profiling of seed-associated bacteria and fungi reveals antagonistic interactions between microorganisms within a shared epiphytic microbiome on Triticum and Brassica seeds.The New phytologist · 2014Article
- Article
- Characterization of the vaginal microbiota of healthy Canadian women through the menstrual cycle.Microbiome · 2014Article
- mPUMA: a computational approach to microbiota analysis by de novo assembly of operational taxonomic units based on protein-coding barcode sequences.Microbiome · 2013Article
- Article
- Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis.Journal of clinical microbiology · 2012Article
- Self-collected vaginal swabs for the quantitative real-time polymerase chain reaction assay of Atopobium vaginae and Gardnerella vaginalis and the diagnosis of bacterial vaginosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial vaginosis (BV) is a recurrent condition that is associated with a range of negative outcomes, including the acquisition of human immunodeficiency virus and other sexually transmitted diseases, preterm births, and pelvic inflammatory disease. In contrast to the Lactobacillus-dominated normal vaginal microbiota, BV is characterized by a lack of lactobacilli and an abundance of anaerobic and gram-negative organisms, including Gardnerella vaginalis and Atopobium vaginae. To date, the laboratory diagnosis of BV has relied upon the fulfillment of criteria determined by microscopic observation of Gram-stained vaginal swabs. We describe a molecular-based method for the easy determination of the species profile within the vaginal microbiota based on the amplification of the chaperonin-60 genes of all bacteria present in the swab and hybridization of the amplicon to species-specific oligonucleotide-coupled fluorescent beads that are identified by flow cytometry with a Luminex instrument. We designed a nineplex Luminex array for characterization of the vaginal microbiota and applied it to the analysis of vaginal swabs from individuals from Africa and North America. Using the presence of A. vaginae or G. vaginalis, or both, as the defining criterion for BV, we found that the method was highly specific and sensitive for the diagnosis of BV using microscopy as a gold standard.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.